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Glu A1 Glu D1位点高分子量谷蛋白亚基共同缺失对弱筋小麦品质的影响
引用本文:武 茹,张 晓,高德荣,别同德,吴荣林,程顺和. Glu A1 Glu D1位点高分子量谷蛋白亚基共同缺失对弱筋小麦品质的影响[J]. 麦类作物学报, 2011, 31(3): 450-454
作者姓名:武 茹  张 晓  高德荣  别同德  吴荣林  程顺和
作者单位:(1.扬州大学农学院,江苏省作物遗传生理重点实验室,教育部植物功能基因组学重点实验室,江苏扬州 225009;2.江苏里下河地区农业科学研究所,江苏扬州 225007)
基金项目:国家重点基础研究发展计划(973计划)项目(2009CB118300);农业部小麦现代农业产业技术体系项目(nycytx03)。
摘    要:为了研究高分子量谷蛋白亚基(HMWGS)缺失对小麦品质的影响,以 GluA1 GluD1位点HMWGS共同缺失材料2GS041410作供体亲本,以弱筋小麦品种扬麦13和扬麦18作轮回亲本进行回交,构建不同遗传背景的BC1F3和BC2F3群体,测定受体、供体亲本的品质,以及回交群体BC1F3和BC2F3 GluA1 GluD1位点HMWGS共同缺失纯合单株及两位点均正常表达纯合单株的品质。结果表明,供体2GS041410具有较低的SDS沉降值、较短的面团形成时间和稳定时间;在BC1F3和BC2F3中, GluA1 GluD1位点HMWGS共同缺失对蛋白含量影响不显著,但可显著或极显著降低SDS沉降值和水溶剂保持力(SRC)。 GluA1 GluD1位点HMWGS双缺失在弱筋小麦品质育种中具有一定的应用价值。

关 键 词:小麦   GluA1   GluD1   HMWGS   双缺失   品质.

Effect of Double deletion of Glu A1 and Glu D1 Locus of High Molecular Weight Glutenin Subunits on Wheat Quality
WU Ru,ZHANG Xiao,GAO De rong,BIE Tong de,WU Rong lin,CHENG Shun he. Effect of Double deletion of Glu A1 and Glu D1 Locus of High Molecular Weight Glutenin Subunits on Wheat Quality[J]. Journal of Triticeae Crops, 2011, 31(3): 450-454
Authors:WU Ru  ZHANG Xiao  GAO De rong  BIE Tong de  WU Rong lin  CHENG Shun he
Abstract:To investigate the effect of HMW GS absence on common wheat quality, the wheat line 2GS0414 10 with double deletion of Glu A1 and Glu D1 locus of HMW GS was used as donor parent in this study. Two soft wheat varieties Yangmai 13 and Yangmai 18 were used as recurrent parents to generate BC1F3 and BC2F3 progenies. Quality test results of the receptor and donor parents, the BC1F3 and BC2F3 progenies showed that the donor 2GS0414 10 had a relatively lower SDS sedimentation value, shorter development time and stability time. Quality evaluation of the backcross progenies indicated that double deletion of Glu A1 and Glu D1 locus significantly influence the SDS sedimentation value and water solvent retention capacity but the protein content. In general, the effect of double deletion of Glu A1 and Glu D1 locus of HMW GS was promising in breeding for quality improvement in soft wheat.
Keywords:Wheat   Glu A1   Glu D1   HMW GS   Double deletion   Quality
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