荧光PCR检测副溶血性弧菌致病基因方法的建立

建立一种准确、快速、特异地检测副溶血性弧菌(Vibrio parahaemolyticus,VP)致病基因的荧光PCR方法。方法 用7株已知其致病基因的VP标准菌株和检出菌分别对6种VP致病基因荧光PCR检测文献方法和本文建立的方法进行验证比较,并用6种常见食源性病原菌对新建方法的特异性进行检验。结果 所建方法能够同时检测VP的3种溶血素,其检测结果与菌株的溯源结果及普通PCR结果完全一致,且与常见食源性病原菌无交叉反应。结论 本文建立的荧光PCR体系能够准确、快速、特异地检测VP的致病基因,具有较好的实用性。

Establishment of a fluorescent PCR method for pathogenic gene Vibrio parahaemolyticus strains

To establish an accurate, rapid and specific fluorescent PCR method to detect pathogenic gene of Vibrio parahaemolyticus(VP).Methods7VP standard stains were used to verify 6literary methods of fluorescent PCR and the new method establish in this study. The specificity was verified using 6common foodborne pathogenic bacteria. ResultsThe newly-established method could detect all 3kinds of hemolysins simultaneously, and the results were consistent with those of stains tracing and standard PCR methods. There was no cross reaction with common foodborne pathogens.ConclusionThe fluorescent PCR system could detect pathogenic gene of VP with better accuracy, reliability, speed and specificity, which was more applicable.