纳豆素对大鼠腹主动脉血栓形成的抑制作用

目的 :在复制大鼠血栓模型上 ,通过动态观察血压的变化、比较血栓的大小以及检测纤溶酶原激活物抑制物 (PAI)、组织型纤溶酶原激活物 (tPA)活性等指标 ,观察纳豆素的溶栓效应。方法 :S -D大鼠随机分五组 (n =40 ) ,用不同浓度的纳豆素灌胃 ,同等剂量的蚓激酶设为阳性对照 ,用Powerlab/ 4s生理多导仪描记股动脉血压 ,血栓干重用考马斯亮蓝法蛋白定量 ,采用酶联免疫法测定血浆中PAI及tPA活性。结果 :纳豆素组与对照组、单纯血栓组相比 ,复制血栓前血压无显著性差异 (P >0 .0 5) ,血栓复制后 40min ,纳豆素组、蚓激酶组血压下降幅度 (-1 1 .62± 7.36 %、- 8.37± 1 0 .2 9%、- 7.2 8± 4 .54 % )与单纯血栓组血压下降幅度 (- 59.97± 1 2 .2 9% )相比具有显著性差异 (P <0 .0 1 ) ;纳豆素处理的动物与单纯血栓组动物比较 ,其血栓的湿重、干重、蛋白含量显著性降低 (P <0 .0 1 ) ;纳豆素组、蚓激酶组血浆中tPA活性升高 ,PAI/tPA比值与对照组相比显著降低 (P <0 .0 1 )。结论 :纳豆素抑制动脉血栓的形成 ,提高纤维蛋白溶解活性

The effect of Nattokinase on the thrombosis formation of abdominal aorta in rat

Objective To study the effect of Nattokinase on the thrombus formation in the thrombus model, the change of blood pressure, the size of thrombus and the activity of plasminogen activator inhibitor(PAI) or tissue plasminogen activator(tPA) were measured.Methods Before making thrombus model by coating the abdomial aorta with filter paper soaked in 30% FeCl 3, S-D rats were divided into five groups randomly, fed with Nattokinase or Lumbrukinaseor normal chow respectively for two days. The femoral artery pressures were traced by Powerlab/4s .The protein contents of the thrombus were determined by Bradford method. The PAI and tPA activity were detected by ELISA. Results The decreased level of femoral artery pressure of the rats fed with Nattokinase or Lumbrukinase were lower (-11.62±7.36%、-8.37±10.29%、-7.28±4.54%)than that of the rats fed with normal chow (-59.97±12.29%, P <0.01). The wet weight, dry weight or the protein content of the thrombus were significantly lower on the rats fed with Natto than that on the rats fed with normal chow ( P <0.01). The tPA activity increased while the PAI/tPA ratio decreased significantly in plasma of the rats fed with Nattokinase or Lumbrukinase, compared with that in plasma of the rats fed with normal chow( P <0.01). Conclusion Nattokinase can inhibit the formation of thrombus and promote the fibrinolysis acitivity in plasma.