HPLC法拆分氟比洛芬对映体

建立氟比洛芬手性药物的高效液相色谱拆分方法。方法:手性流动相添加剂HPLC法:利用C18柱,以羟丙基-β-环糊精作为手性流动相添加剂,调节有机修饰剂甲醇的比例和添加不同量的三乙胺对氟比洛芬进行拆分;手性固定相HPLC法:利用Chiral-pakAD手性柱,以正己烷-乙腈为流动相基本成分,调整两者不同比例和添加不同量的三乙胺,对氟比洛芬进行拆分。结果:手性流动相添加剂法:使用C18柱对氟比洛芬对映异构体进行拆分,调节流动相中有机修饰剂甲醇浓度、手性流动相添加剂羟丙基环糊精浓度、峰型修饰剂三乙胺的浓度等都不能使氟比洛芬对映体达到基线分离,只能部分分离。手性固定相法:氟比洛芬对映体在Chiral-pakAD手性柱上能达到较好的分离。在正己烷-乙腈流动相系统中,正己烷体积含量为90%,三乙胺体积含量为0.05%的条件下,氟比洛芬对映体得到了较好的分离,分离度为10.0。结论:建立的手性固定相法能有效拆分氟比洛芬对映体而手性流动相添加剂法不能拆分氟比洛芬对映体。

Chromatographic Separation and Enantiomeric Resolution of Flurbiprofen by HPLC Method

Objective:To establish chiral HPLC methods for the enantiomeric separation of flurbiprofen.Methods:On the one hand,enantiomeric separation of flurbiprofen was performed on a C18 column using hydroxypropyl-β-cyclodextrin as a chiral mobile phase additive(CMPA)with the mobile phase of consisting n-hexane,methanol and proporation of triethylamine(TEA);On the other hand,enantiomeric separation of flurbiprofen was performed on a chiral-pak AD column with the mobile phase of consisting n-hexane,acetonitrile and proporation of triethylamine(TEA).The mobile phase compositions were-systematically investigated to obtain the optimal chromatographic profile.Results:The use of HP-β-CD as a CMPA for the enantiomeric resolution of flurbiprofen proved to be of little success with none of the analytes being baseline resolved.Enantiomeric resolution of 10.0 was obtained for flurbiprofen on a chiral-pak AD column using a mobile phase of n-hexane:acetonitrile(90:10v/v)containing triethylamine(0,05%v/v).Conclusion:Flurbiprofen are separated successfully by the chiral stationary phase method other than the chiral mobile phase additive method.