华支睾吸虫成虫特异性诊断抗原分析

目的:寻找诊断华支睾吸虫病的特异性抗原.方法:应用SDS-PAGE和Western blot对华支睾吸虫成虫可溶性抗原中的蛋白组分进行分析,试验血清包括旋毛虫感染的大鼠和小鼠血清,正常大鼠和小鼠血清,旋毛虫病、华支睾吸虫病、并殖吸虫病、日本血吸虫病及囊虫病患者血清,斯氏狸殖吸虫感染的大鼠血清和正常人血清.结果:华支睾吸虫成虫可溶性抗原蛋白经SDS-PAGE后显示17条蛋白带,其中相对分子质量为65 000、54 000、31 000、25 000、13 000的为主带.Western blot结果显示,相对分子质量为108 000、94 000、71 000、65 000、56 000、53 000、43 000的蛋白带可被华支睾吸虫病患者血清识别,108 000、71 000、65 000、63 000、53 000的蛋白带可被并殖吸虫病患者血清识别,108 000、94 000、71 000可被日本血吸虫病患者血清、旋毛虫感染的大鼠血清及旋毛虫病患者血清识别;56 000的蛋白组分只能被华支睾吸虫病患者血清识别,而不与其他试验血清发生交叉反应.结论:华支睾吸虫成虫可溶性抗原中相对分子质量为56 000的蛋白组分为华支睾吸虫成虫的特异性抗原.

Analysis of specific diagnostic antigens of Clonorchis sinensis adult worms

Aim: To find out the specific antigens for immunodiagnosis of clonorchiasis. Methods: The soluble antigens of Clonorchis sinensis(C.sinensis) adult worms were analyzed by SDS-PAGE and Western blot.Sera for this study included sera from patients with clonorchiasis, paragonimiasis, schistosomiasis, trichinellosis,sera from rats and mice infected with T.spiralis,and sera from normal rats,mice and persons. Results: SDS-PAGE revealed that the soluble antigens of C.sinensis adult worms had 17 protein bands. Western blot results showed that the protein bands with MW 108 000, 71 000, 65 000,63 000,53 000 were cross-reacted with sera from patients with paragonimiasis. All of the protein bands with MW 108 000,94 000,71 000 were cross-reacted with sera from patients with schistosomiasis, rats infected with T.spiralis and patients with trichinellosis. The protein component with MW 56 000 was only reacted with sera from patients with clonorchiasis.Conclusion: The protein component with MW 56 000 is the specific antigen for C.sinensis adult worm.