| 高效液相色谱-蒸发光散射法测定马鞭草中齐墩果酸与熊果酸的含量 |
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| 引用本文： | 丁家欣,张秋海,李先端,李淑莉,刘泓. 高效液相色谱-蒸发光散射法测定马鞭草中齐墩果酸与熊果酸的含量[J]. 分析仪器, 2011, 0(5): 26-29 |
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| 作者姓名： | 丁家欣 张秋海 李先端 李淑莉 刘泓 |
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| 作者单位： | 1. 中国中医科学院中医基础理论研究所
2. 中国中医科学院中药研究所,北京,100700 |
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| 摘 要： | 建立了高效液相色谱一蒸发光散射检测法测定马鞭草药材中齐墩果酸和熊果酸含量的方法，色谱柱为PinnacleⅡ C18（250min×4．6mm，5m），流动相为甲醇-0．2％冰醋酸（体积比82．5：17．5）；淋洗速度1mL／min；柱温35℃；蒸发光散射检测器检测，漂移管温度为85℃；气体流速为2．2L／min。齐墩果酸在0．317～1．585μg范围内（r=0．9997），熊果酸在0．585～2．925g范围内（r=0．9999）线性关系良好。药材中齐墩果酸和熊果酸的回收率（n=6）分别为100．4％和97．6％，标准偏差分别为1．84％和2．34％。所建方法准确，重现性好，可作为马鞭草药材的质量控制方法。
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| 关 键 词： | 马鞭草 齐墩果酸 熊果酸 高效液相色谱-蒸发光散射检测法 |
HPLC-ELSD determination of oleanolic acid and ursolic acid in Verbena officinalis L |
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| Ding Jiaxin,Zhang Qiuhai,Li Xianduan,Li Shuli,Liu Hong. HPLC-ELSD determination of oleanolic acid and ursolic acid in Verbena officinalis L[J]. Analytical Instrumentation, 2011, 0(5): 26-29 |
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| Authors: | Ding Jiaxin Zhang Qiuhai Li Xianduan Li Shuli Liu Hong |
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| Affiliation: | 1. Institute of Chinese Medicine Basic Theory, Academy of Traditional Chinese Medicine, Beijing, 100700; 2. Institute of Chinese Materia Medica, Academy of Traditional Chinese Medicine, Beijing, 100700) |
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| Abstract: | A method for the determination of oleanolic acid and ursolic acid in Verbena officinalis L by high performance liquid chromatography-evaperative light scattering detector （HPLC-ELSD） was established. A Pinnacle Ⅱ C18 column （4.6mm×250mm） was used for separation at 35℃. The mobile phase was methanol-0.2% acetic acid （82.5 ： 17.5） with the flow rate of lmL/min. The drift tube temperature was 85℃ and the gas flow rate was 2. 2L/min. The oleanolic acid had a good linearity in the range of 0. 317 1. 585μg （ r =0. 997） and ursolic acid in the range of 0. 585-2. 925μg （ r ：0. 9999）. The average recoveries of oleanolic acid and ursolic acid were 100.4% （RSD=1.84%） and 97.6% （RSD=2.34%）, respectively. The method is accurate and repeatable and could be used for the quality control of Verbena officinalis L. |
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