Development of an automated production process of [64Cu][Cu (ATSM)] for positron emission tomography imaging and theranostic applications |
| |
Authors: | Tengzhi Liu Kathrine Røe Redalen Morten Karlsen |
| |
Affiliation: | 1. Department of Physics, Norwegian University of Science and Technology, Trondheim, Norway;2. Department of Radiology and Nuclear Medicine, St. Olavs Hospital, Trondheim University Hospital, Trondheim, Norway |
| |
Abstract: | Cyclotron-produced copper-64 radioisotope tracers offer the possibility to perform both diagnostic investigation by positron emission tomography (PET) and radiotherapy by a theranostic approach with bifunctional chelators. The versatile chemical properties of copper add to the importance of this isotope in medicinal investigation. 64Cu]Cu (ATSM)] has shown to be a viable candidate for imaging of tumor hypoxia; a critical tumor microenvironment characteristic that typically signifies tumor progression and resistance to chemo-radiotherapy. Various production and radiosynthesis methods of 64Cu]Cu (ATSM)] exist in labs, but usually involved non-standardized equipment with varying production qualities and may not be easily implemented in wider hospital settings. 64Cu]Cu (ATSM)] was synthesized on a modified GE TRACERlab FXN automated synthesis module. End-of-synthesis (EOS) molar activity of 64Cu]Cu (ATSM)] was 2.2–5.5 Ci/μmol (HPLC), 2.2–2.6 Ci/μmol (ATSM-titration), and 3.0–4.4 Ci/μmol (ICP-MS). Radiochemical purity was determined to be >99% based on radio-HPLC. The final product maintained radiochemical purity after 20 h. We demonstrated a simple and feasible process development and quality control protocols for automated cyclotron production and synthesis of 64Cu]Cu (ATSM)] based on commercially distributed standardized synthesis modules suitable for PET imaging and theranostic studies. |
| |
Keywords: | 64Cu-ATSM automated synthesis hypoxia PET radiolabeling Tracerlab |
|
|