白细胞介素-1B 基因多态性、幽门螺杆菌感染与胃癌发生的相关性

 目的 探讨湖南衡阳地区白细胞介素-1B（IL-1B）基因多态性与胃癌的关系以及幽门螺杆菌（Helicobacter pylori,HP）感染后胃癌发生的易感基因型。方法 52例胃癌患者癌旁正常胃粘膜组织和55例慢性胃炎患者胃粘膜组织,均经快速尿素酶和PCR检测HP,应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析技术,进行基因型检测,并对C/C、T/T进行测序,比较各基因型在胃癌组和胃炎组中的分布差异。结果 IL-1B-31T、IL-1B-511T等位基因和IL-1B-31T/T、IL-1B-511T/T基因型在胃癌组的分布频率高于胃炎组（P〈0.05）,OR值分别为1.97（95%CI=1.15-3.59）、2.52（95%CI=1.45-4.39）和2.71（95%CI=1.10-6.66）、3.33（95%CI=1.14-9.73）。在伴有HP感染的群体中进行比较,IL-1B-31位点各基因型未见明显差异;但IL-1B-511T等位基因和IL-1B-511T/T基因型在胃癌组的分布频率高于胃炎组（P〈0.05）,OR值分别为2.16（95%CI=1.10-4.23）和3.43（95%CI=1.01-11.62）。结论 在湖南衡阳地区IL-1B-31T/T、IL-1B-511T/T基因型与胃癌发病风险相关,在HP被感染后IL-1B-511T/T基因型可能为湖南衡阳地区胃癌易感基因型。

Relation of Interleukin-1B Gene Polymorphism and Helicobacter Pylori Infection to Gastric Cancer

Objective 　To investigate the association of the interleukin-1B ( IL-1B) gene polymorphism with gastric cancer in Henyang Chinese population ,and to study which type may be the sensitive genotype to gastric cancer in patients with helicobacter pylori ( HP) infection. Methods 　For 52 patients with gastric cancer and 55 patients with chronic gastritis. ,the polymorphism of the IL-1B gene was studied by using polymerase chain reaction-rest riction f ragment length polymorphism ( PCR-RFL P) analysis , C/ C、T/ T genotype were sequenced. The infections of HP were determined by RU T and PCR assay. Results 　The frequencies of IL-1B-31 T , IL-1B-511 T , IL-1B-31 T/ T and IL-1B-511 T/ T genotype were higher in gastric cancer group than in chronic gast ritis group ( P < 0. 05) , with odds ratios (ORs) of 1. 97 (95 %CI = 1. 15～3. 59) , 2. 52 (95 %CI = 1. 45～4. 39) ,2. 71 (95 %CI = 1. 10～6. 66) and 3. 33 (95 %CI = 1. 14～9. 73) ,respectively. In patient s with HP infections , the IL-1B-511 T and IL-1B-511 T/ T were higher in gastric cancer group than in chronic gastritis group ( P < 0. 05) , with odds ratios of 2. 16 (95 %CI = 1. 10～4. 23) and 3. 43 (95 %CI = 1. 01～11. 62) , respectively. Conclusion 　In the HengYang Chinese population , the IL-1B-31 T/ T and IL-1B-511 T/ T genotypes may be associated with the high risk of gastric cancer , and IL-1B-511 T/ T may be the sensitive genotype of gastric cancer in patients with HP infection.