大叶千斤拔根的化学成分

目的 研究大叶千斤拔根的化学成分.方法 采用柱色谱及薄层色谱进行分离纯化,根据其理化性质及波谱数据鉴定化学结构.结果 分离得到11个化合物,其中异黄酮4个,双氢黄酮3个,黄烷醇1个,分别是染料木素(I)、5,7.3',4'-四羟基异黄酮(II)、5,7,4'-三羟基异黄酮-7-O-β-D吡喃葡萄糖苷(III)、5,7,4'-三羟基-8,3'-二异戊烯基双氢黄酮(IV)、5,7,4'-三羟基-6-异戊烯基异黄酮(V)、云南千斤拔素(flemichin D,VI)、胡枝子黄烷酮A(lespedezaflavanone A,Ⅶ)、赛金莲木儿茶精(ouratea-catechin,Ⅷ)、3.4,5-三甲氧基苯-O-β-D-葡萄糖苷(Ⅸ)、豆甾醇-3-O-β-D-吡喃葡萄糖苷(X)、豆甾醇(XI).结论 化合物Ⅲ～V、Ⅶ～Ⅺ为首次从该种植物中得到.化合物I～Ⅺ为首次从该植物根中得到,其中以生理活性成分染料木素为代表的异黄酮为该植物根的主要成分.

Chemical constituents in roots of Flemingia macrophylla

Objective To study the chemical constituents in the roots of Flemingia macrophylla. Methods To separate and purify compounds by column chromatography and TLC, and to determine their chemical structures by their physical characters and spectral data. Results Eleven compounds were purified from the extraction in the roots of F. Macrophylla, among them four are isoflavones, three are flava-nones, and one is flavanol. They are genistein ( I ), orobol ( Ⅱ ), 5, 7, 4'trihydroxyisoflavone-7-O-D-glucopyranoside (Ⅲ ), 5, 7, 4'-trihydroxy-8, 3'-diprenylflavanone (Ⅳ ) , 5, 7, 4'-trihydroxy-6-prenyli-soflavone (V), flemichin D (Ⅵ), lespedezaflavanone A(Ⅶ), ouratea-catechin (Ⅷ), 3, 4, 5-trimethoxy benzene-O-β-D-glucopyranoside (Ⅸ), stigmasterol-3-O-β-D-glucopyranoside (X),and stigmasterol (XI). Conclusion Compounds Ⅲ -V, and Ⅶ-Ⅺ are found from this plant for the first time. All the com-pounds are found from the roots of this plant for the first time. The active components, genistein and its isoflavone analogs, are main constituents in the roots of F. Macrophylla.