淋巴组织增生性疾病组织中EB病毒的原位杂交检测

目的探讨ＥＢ病毒与我国各类淋巴组织增生性疾病的关系。方法以ＥＢ病毒ＬＭＰ基因为探针,对２１４例淋巴组织增生性疾病组织中ＥＢ病毒进行原位杂交检测,并用ＳＹＳＴＡＴ软件对实验结果进行分析。结果霍奇金淋巴瘤（ＨＤ）、非霍奇金淋巴瘤（ＮＨＬ）、淋巴组织良性增生（ＢＬＰ）组织中ＥＢ病毒阳性率分别为３０．０％（１５／５０）,１４．０％（１８／１２９）及２．９％（１／３５）。在ＮＨＬ中,高度恶性（ＨＮＨＬ）、中度恶性（ＭＮＨＬ）及低度恶性（ＬＮＨＬ）ＥＢ病毒阳性率分别为２８．１％（９／３２）、１０．５％（９／８４）及０％（０／９）。ＨＤ与ＨＮＨＬ间ＥＢ病毒阳性率均显著高于ＢＬＰ（Ｐ＜０．０１,Ｐ＜０．０５）,ＭＮＨＬ和ＬＮＨＬ与ＢＬＰ间ＥＢ病毒阳性率差异无显著性（Ｐ＞０．０５）。结论ＥＢ病毒与ＨＤ及ＨＮＨＬ的发生有关,而与ＭＮＨＬ及ＬＮＨＬ的发生关系不大。

Detection of Epstein-Barr virus in lymphoproliferative diseases by in situ hybridization

Objective To study the relationship between Epstein Barr virus and various lymphoproliferative diseases in China. Methods Tissues from 214 patients with lymphoproliferative diseases were examined for the presence of Epstein Barr virus (EBV) using in situ hybridization with EBV's LMP gene as probe. Results The positive rate of EBV in Hodgkin's disease(HD), Non Hodgkin's Lymphoma(NHL), benign lymphoid proliferative disease(BLP) was 30.0%(15/50),14.0%(18/129),2.9%(1/35), respectively. Among NHL, EBV was detected in 28.1%(9/32) of high grade lymphoma(HNHL),10.5%(9/84) of medium grade lymphoma(MNHL) and in none(0/9) of low grade lymphoma(LNHL).Significantly higher rates than that of BLP were observed in HD and HNHL.No significant differences in rates among patients with MNHL,LNHL and BLP. Conclusion EBV may be involved in the pathogenesis of HD and HNHL, but not in MNHL and LNHL.