樱桃种质SCoT分子标记与叶片表型性状关联分析

利用本研究中建立的樱桃SCoT标记分析体系,进行适宜引物筛选,进而对12个樱桃品种进行SCoT分子标记、叶片表型性状遗传多样性及两者关联分析。结果显示:从102条SCoT标记引物中筛选出46条适宜樱桃遗传分析的标记引物,筛选率达45.1%;参试的18条SCoT引物共获得等位位点数77个,其中多态性位点65个,多态性比率84.4%;12个樱桃品种的表型叶片性状变异系数为11.49%~36.93%,叶片性状与SCoT标记聚类分析结果基本一致;Pearson’s相关分析表明,共有54个标记位点与12个性状呈显著或极显著相关;多元线性逐步回归分析获得了8个与叶片性状极显著相关的特异标记位点,解释了叶片性状47.8%~100.0%的变化;对8个关联标记进行序列比对分析,有5个标记能在樱桃基因组中找到同源序列,相似性均达75%以上,并能明确其染色体位置,其中4个标记能够在NCBI中比对到具有功能注释的同源序列,另3个标记未比对到同源序列。

Association Analysis of SCoT Markers and Leaf Phenotypic Traits in Cherry Germplasm

Based on the established SCoT analysis system,the suitable primers were screened,and then SCoT molecular markers,genetic diversity of leaf traits and their correlation were analyzed in 12 cherry varieties.The results showed that 46 marker primers being suitable for cherry genetic analysis were selected from 102 SCoT marker primers,with a screening rate of 45.1%.A total of 77 allele points were obtained from 18 SCoT primers,including 65 polymorphic loci,with a polymorphism rate of 84.4%.The variation coefficient of leaf traits of 12 varieties was 11.49%-36.93%,which was basically consistent with the results of SCoT marker clustering analysis.Pearson’s correlation analysis showed that a total of 54 marker sites were significantly or extremely significantly correlated with 12 leaf traits.Through the multiple linear stepwise regression analysis,eight specific marker sites related to leaf traits were obtained,which explained the change of 47.8%-100.0%of leaf traits.Sequence analysis of eight association marker sites showed that five markers could find homologous sequences in cherry genome;the similarity was more than 75%;and their chromosome positions could be identified.Among them,four markers could find homologous sequences with functional annotation in NCBI,and three markers could not find homologous sequences.