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Genetically modified starter and protective cultures
Authors:R Geisen  WH Holzapfel
Affiliation:

Federal Research Centre for Nutrition, Institute of Hygiene and Toxicology, D-76131, Karlsruhe, Germany

Abstract:Modern approaches towards starter and protective culture improvement rely on advances in molecular biology. For most microorganisms used for food production, gene technological methods have been well developed. By recombinant DNA technology, ‘tailor-made’ starter and protective cultures may be constructed so as to combine technically desirable features. A single strain which normally would fail to accomplish a given ‘wtask’ may now be improved so as to meet a set of requirements necessary for a specific production or preservation process (e.g. wholesomeness, no off-flavour production, overproduction of bacteriocins or particular enzymes). In addition, undesirable properties (e.g. mycotoxin or antibiotic production by cheese moulds) may be eliminated by techniques such as ‘gene disruption’.
Keywords:Lactic acid bacteria (LAB)  Yeasts  Fungi  Genetic improvement  Transformation systems  Heterologous gene expression
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