| 群体感应在Pseudomonas aeruginosa生物强化反硝化中的应用研究 |
| |
| 引用本文： | 阮心怡,崔雪燕,殷峻,李娜,汪美贞,沈东升. 群体感应在Pseudomonas aeruginosa生物强化反硝化中的应用研究[J]. 环境科学学报, 2020, 40(5): 1661-1666. DOI: 10.13671/j.hjkxxb.2019.0459 |
| |
| 作者姓名： | 阮心怡 崔雪燕 殷峻 李娜 汪美贞 沈东升 |
| |
| 作者单位： | 浙江工商大学,环境学院,杭州310012;浙江省固体废物处理与资源化重点实验室,杭州310012,浙江工商大学,环境学院,杭州310012;浙江省固体废物处理与资源化重点实验室,杭州310012,浙江工商大学,环境学院,杭州310012;浙江省固体废物处理与资源化重点实验室,杭州310012,浙江工商大学,环境学院,杭州310012;浙江省固体废物处理与资源化重点实验室,杭州310012,浙江工商大学,环境学院,杭州310012;浙江省固体废物处理与资源化重点实验室,杭州310012,浙江工商大学,环境学院,杭州310012;浙江省固体废物处理与资源化重点实验室,杭州310012 |
| |
| 基金项目： | 浙江省自然科学基金项目（No.LY17E080001，LQ18E080005） |
| |
| 摘 要： | 采用模拟废水研究了群体感应对反硝化菌株定植（即生物强化作用）及反硝化作用的影响.反硝化功能基因napA表达结果表明，菌株Pseudomonas aeruginosa能够在反应器中成功定植.通过对不同反应器中信号分子的测定发现，群体感应有利于菌株的定植，硝酸盐的去除效率由55%±3.0%提升至81%±3.8%.在菌株定植成功的反应器中加入群体感应淬灭酶后，信号分子失活，促使反应器中硝酸盐的去除率进一步提升至95%以上.
|
| 关 键 词： | 群体感应 生物强化 反硝化 废水处理 |
| 收稿时间： | 2019-10-27 |
| 修稿时间： | 2019-12-05 |
Application of quorum sensing in bioaugmentation for denitrification process with Pseudomonas aeruginosa |
| |
| RUAN Xinyi,CUI Xueyan,YIN Jun,LI N,WANG Meizhen and SHEN Dongsheng. Application of quorum sensing in bioaugmentation for denitrification process with Pseudomonas aeruginosa[J]. Acta Scientiae Circumstantiae, 2020, 40(5): 1661-1666. DOI: 10.13671/j.hjkxxb.2019.0459 |
| |
| Authors: | RUAN Xinyi CUI Xueyan YIN Jun LI N WANG Meizhen SHEN Dongsheng |
| |
| Affiliation: | 1. School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012;2. Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012,1. School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012;2. Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012,1. School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012;2. Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012,1. School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012;2. Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012,1. School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012;2. Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012 and 1. School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012;2. Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012 |
| |
| Abstract: | The effect of quorum sensing on bacterial colonization (bioaugmentation) and denitrification were investigated with synthetic wastewater. The results of denitrification gene (napA) expression showed that the strain Pseudomonas aeruginosa was successfully colonized in the reactors. By measuring the concentrations of signal molecules in different reactors, it was found that quorum sensing was beneficial to the colonization of the strains. The nitrate removal in the reactor increased from 55%±3.0% to 81%±3.8% after the bioaugmentation. When quorum quenching enzyme was added into the reactors which contained colonized strains, the signals were deactivated, and the nitrate removal was further enhanced to more than 95%. |
| |
| Keywords: | quorum sensing bioaugmentation denitrification wastewater treatment |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《环境科学学报》浏览原始摘要信息 |
|
点击此处可从《环境科学学报》下载全文 |
|
