复方杠柳液外用治疗尖锐湿疣临床观察及对离体人乳头瘤病毒DNA的影响

目的观察中药复方杠柳液治疗尖锐湿疣(condyloma acuminatum，CA)的临床疗效，并从分子水平探讨其作用机理。方法将81例尖锐湿疣患者，随机分为复方杠柳液(30例)、杠柳浆(21例)、尤脱欣(30例)3组，评定各组疗效及不良反应发生率。采用聚合酶链反应技术(polymerase chain reaction，PCR)，检测2例离体尖锐湿疣疣体中人乳头瘤病毒(human papilloma virus，HPV)DNA经复方杠柳液及其君药杠柳浆作用后的变化。结果复方杠柳液、杠柳浆、尤脱欣3组的痊愈率分别为56．67％、42．86％、63．33％：总有效率分别为83．33％、71．43％、86．67％，3组疗效比较差异无显著性。复方杠柳液、杠柳浆、尤脱欣3组的不良反应发生率分别为6．67％、4．76％、86．67％，差异有显著性(P<0．01)；复发率分别为14．3％、12．5％、47．1％。两份不同及相同浓度疣体匀浆混悬液经复方杠柳液、杠柳浆作用后PCR均阴性，未经药物处理的生理盐水组PCR均阳性。结论复方杠柳液、杠柳浆、尤脱欣3组最终疗效相近，复方杠柳液及杠柳浆组不良反应少、复发率低。该药对离体CA组织中的HPV—DNA可能有破坏作用。

Clinical Efficacy of Compound Periploca Liquid in Treating Condyloma Acuminatum and Its Effect on Human Papilloma Virus DNA Expression in vitro

ObjectiveTo investigate the clinical efficacy of compound periploca liquid (CPL) in treating condyloma acuminatum (CA), and to explore its mechanisms at molecular level. MethodsEighty-one patients with CA were randomly divided into three groups, 30 patients in Group A were treated with CPL, 21 in Group B were treated with periploca syrup and 30 in Group C were treated with Youtuoxin (YXG). The clinical efficacy and adverse reaction occurred in the three groups was evaluated respectively. Besides, change of human papilloma virus (HPV) DNA in two patients with CA was determined by polymerase chain reaction (PCR) in vitro after being treated with CPL and periploca, the monarchic drug of CPL. ResultsThe cure rate obtained in group A, B, and C was 56.67%, 42.86% and 63.33% respectively, the total effective rate in them was 83.33%, 71.43% and 86.67% respectively, the difference of therapeutic efficacy was insignificant among the three groups. But the adverse reaction occurrence in them (6.67%, 4.76% and 86.67%) was significantly different (P<0.01). The recurrent rate in them was 14.29%, 12.5% and 47.1% respectively. PCR showed negative expression of HPV in the two samples of CA of same concentration after the verrucous homogenate suspension being treated with CPL or periploca syrup of different concentration, while it was positive after the suspension was treated with the group of normal saline without any drug. ConclusionThe therapeutic efficacies of CPL, periploca syrup and Youtuoxin are not significantly different, but the former two have advantages of less adverse effects and lower recurrent rate. And they are possibly having germicidal action on HPV-DNA in CA tissue in vitro.