苹果茎沟病毒吉林沙果分离物全基因组序列分析

通过RT-PCR结合RACE技术扩增到苹果茎沟病毒（Apple stem grooving virus，ASGV）吉林沙果分离物（ASGV-JLSG）全长基因组（登录号：FM616381），共含有6 496个核苷酸（nt），编码两个彼此重叠的开放阅读框（Open reading frame，ORF）。ORF1（37 ~ 6 354 nt）编码1个241 kD的多聚蛋白，含有甲基转移酶（Methyltransferase）、木瓜蛋白酶（Papain-like-protease）、解旋酶（Nucleotide triphosphate-binding helicase）、RNA依赖的RNA聚合酶（RNA-dependent RNA polymerase）和C端的外壳蛋白（Coat protein，CP）等结构域。ORF2（4 788 ~ 5 750 nt）编码1个36 kD的运动蛋白（Movement protein，MP）。ASGV-JLSG分离物与GenBank公布的29个ASGV分离物全基因核苷酸序列一致性为79.20% ~ 86.60%。系统发育分析结果表明，30个ASGV分离物分成2个组，分离物间没有表现出寄主专一性和地理分布规律。重组分析发现，ASGV-JLSG分离物是苹果茎沟病毒黄花梨分离物（ASGV-HH，JN701424）和柑橘碎叶病毒满头红分离物（CTLV-MTHK，C588948）的重组体。本研究中获得的ASGV-JLSG分离物基因组是来源于中国东北地区的首个ASGV基因组序列。

Analysis of the Complete Genome of Apple stem grooving virus Isolate Jilin-shaguo

Using RT-PCR and RACE techniques，the complete genome of Apple stem grooving virus（ASGV）isolate Jilin-shaguo（JLSG）was amplified. The genome of ASGV-JLSG has 6 496 nucleotides，encoding two overlapping open reading frames（ORFs）. The ORF1（position 37–6 354 nt）encodes a 241 kD polyprotein，including domains of a methyltransferase，a papain-like-protease，a nucleotide triphosphate-binding helicase，a RNA-dependent RNA polymerase，and a coat protein. ORF2（4 788– 5 750 nt）encodes a movement protein. The nucleotide identity of complete genome between ASGV-JLSG and the 29 ASGV isolates published in GenBank ranged from 79.20% to 86.60%. Phylogenetic analysis indicated that the concerned ASGV isolates can be grouped into 2 clusters，but no geographic or host association among one another. Recombination analysis suggested that ASGV-JLSG was the recombinant of ASGV-HH（JN701424）and CTLV-MTH（KC588948）. This was the first reported complete genome of ASGV in Northeast China.