腺苷脱氨酶抑制巨噬细胞增殖迁移的作用研究

目的:探讨腺苷脱氨酶对鼠源巨噬细胞RAW264.7增殖、迁移、细胞周期、细胞凋亡的影响。方法:用不同浓度(0、0.25、1.25、2.5、5U/m L)的腺苷脱氨酶处理RAW264.7细胞后,用实时细胞分析系统检测细胞增殖能力,用流式细胞术检测腺苷脱氨酶对细胞凋亡和周期的影响,划痕修复实验检测RAW264.7细胞迁移能力。结果:与对照组相比,高浓度腺苷脱氨酶(2.5 U/m L、5 U/m L)处理可以显著抑制RAW264.7细胞的增殖能力,且抑制效果随腺苷脱氨酶浓度升高而增强(P<0.05)。流式细胞术检测结果显示,相较于对照组,高浓度腺苷脱氨酶(2.5 U/m L)处理可以诱导RAW264.7细胞凋亡,并导致细胞周期G2/M期阻滞(P<0.05)。此外,细胞划痕实验表明,高浓度腺苷脱氨酶(2.5 U/m L)处理可以显著抑制RAW264.7巨噬细胞的迁移能力(P<0.05)。结论:高浓度腺苷脱氨酶对巨噬细胞增殖和迁移具有抑制作用,并可诱导细胞凋亡和细胞周期阻滞。

The Effects of Adenosine Deaminase on Macrophage Cells Proliferation and Migration

ABSTRACT Objective: To investigate the effects of adenosine deaminase on the proliferation, migration, cycle and apoptosis of macrophage RAW264.7 cells. Methods: RAW264.7 cells were treated with adenosine deaminase at gradient concentrations (0, 0.25, 1.25, 2.5, 5U/mL). The proliferation ability was detected by realtimecellularanalysis. The effect of adenosine deaminase on apoptosis and cell cycle was detected by flow cytometry. Scratch repair experiment was used to investigate the migration ability of RAW264.7 cells. Results: Compared with the control group, high concentration of adenosine deaminase (2.5 U/mL, 5 U/mL) significantly inhibited the proliferation capacity of RAW264.7 cells compared with the control group, and the inhibitory effect was enhanced with the increase of adenosine deaminase concentration (P<0.05). Flow cytometry showed that high concentration of adenosine deaminase (2.5 U/mL) induced RAW264.7 cell apoptosis and led to G2/M cell cycle arrest (P<0.05). In addition, cell scratch experiments showed that high concentration of adenosine deaminase (2.5 U/mL) significantly inhibited RAW264.7 macrophage migration (P<0.05). Conclusion: Adenosine deaminase inhibits the proliferation and migration of macrophages and induces apoptosis and cell cycle arrest.