沙棘多酚提取纯化工艺研究

以沙棘冻干粉为原料，采用超声辅助乙醇法提取沙棘多酚，通过单因素试验和响应面试验优化提取条件，并通过大孔树脂对沙棘多酚进行静态吸附-解析试验，选取适合沙棘多酚纯化的大孔树脂，对纯化前后的沙棘多酚进行体外抗氧化试验。结果表明:沙棘多酚的最佳提取工艺为:料液比1∶24 g/mL，乙醇浓度49%，超声时间52 min，超声温度48℃，在此条件下沙棘多酚的提取量为8.61 mgECGC/g；确定AB-8为纯化沙棘多酚适合的大孔树脂，纯化前后清除DPPH自由基的IC₅₀值分别713.22、142.53 μg/mL，纯化前后清除ABTS自由基的IC₅₀值分别为61.92、8.68 μg/mL，经过纯化后的沙棘多酚抗氧化性明显升高。

Study on Extraction and Purification Process of Sea buckthorn Polyphenols

The raw material was sea buckthorn lyophilized powder,and the ultrasonic-assisted and ethanol solvent method was used to extract sea buckthorn polyphenols.The extraction conditions were optimized by single-factor test and response surface test.The macroporous resin was used for static adsorption-analysis test of sea buckthorn polyphenols.In vitro antioxidant tests were carried out on sea buckthorn polyphenols before and after purification.The test results showed that when the ratio of liquid to solid was 1∶24(g/mL),the volume fraction of ethanol was 49%,the extraction time was 52 min,and the extraction temperature was 48℃,the extraction amount of polyphenol was the largest,reaching(8.61±0.25)mgECGC/g.The AB-8 was identified as suitable macroporous resin for purification of sea buckthorn polyphenols.The semi-inhibitory concentrations(IC 50)of polyphenols from sea buckthorn on DPPH free radical before and after purification were 713.22 and 142.53μg/mL,and The semi-inhibitory concentrations(IC 50)of polyphenols from sea buckthorn on ABTS free radical before and after purification were 61.92,8.68μg/mL.The results showed that the polyphenols from sea buckthorn after purification had good antioxidant capacity.