健骨颗粒抗去卵巢大鼠骨质疏松的血清代谢组学研究

目的 运用代谢组学方法从物质代谢角度探讨健骨颗粒治疗绝经后骨质疏松症的机制。 方法 60只大鼠随机分为正常组、模型组和健骨颗粒组，健骨颗粒组给予健骨颗粒药液灌胃，其余组分别给予等量生理盐水灌胃干预。分别于术后6周和12周取股骨、胫骨、腰椎骨进行骨密度及骨生物力学检测，取腹主动脉血进行液相色谱-质谱（LC-MS）检测，综合单变量和多变量统计分析，鉴定并筛选血清代谢物。结果 模型组较正常组骨密度和骨最大载荷显著下降，健骨颗粒组与模型组相比明显恢复，表明造模成功且健骨颗粒组有恢复正常的趋势。模型组血清代谢谱与正常组分离，健骨颗粒组接近正常组。筛选出硬脂酸、皮质酮及泛酸等27个差异代谢物，分别与不饱和脂肪酸、类固醇激素的生物合成及泛酸和辅酶A生物合成等通路有关，涉及脂代谢、核酸代谢及氨基酸代谢等。结论 健骨颗粒可能通过调节多种代谢通路改善绝经后骨质疏松症。

Serum metabonomics study of anti-osteoporosis effect of Jiangu granule in ovariectomized rats

Objective To investigate the mechanism of Jiangu Granule in the treatment of postmenopausal osteoporosis by metabonomics. Methods Sixty rats were randomly divided into normal group, model group and Jiangu Granule group.The Jiangu granule group was given Jiangu granule solution by gavage, and the others were given the same capacity saline by gavage. After 6 week or 12 week, the femur, tibia and lumbar vertebrae were used for the test of bone mineral density(BMD) and biomechanics. The blood of abdominal aorta was analyzed by liquid chromatography-mass spectrometry (LC-MS). After univariate statistics and multivariate statistics analysis, the potential metabolites were selected. Results Compared with the normal group, the BMD and maximum load of model group decreased significantly, while the Jiangu Granule group tended to return to normal level. It was indicated that the model was successful and the rat body was tended to return to normal. The serum metabolic spectrum of the model group and the normal group were completely separated. Jiangu Granule group was close to the normal group. 27 different metabolites, such as pantothenate, stearic acid and corticosterone, were screened out, which were related to the biosynthesis of pantothenate and CoA , unsaturated fatty acids and steroid hormones, respectively. These metabolites were involved in lipid metabolism, nucleic acid metabolism, amino acid metabolism and so on. Conclusion Jiangu granule may treat postmenopausal osteoporosis by regulated multiple metabolic pathways.