A rapid visual method to identify transformed plants |
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Authors: | Martha S Wright Karen Launis Cindy Bowman Martha Hill Joe Dimaio Catherine Kramer Ray D Shillito |
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Affiliation: | (1) Ciba Agricultural Biotechnology, 3054 Cornwallis Road, 27709 Research Triangle Park, North Carolina |
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Abstract: | Summary A rapid, efficient assay that is nondestructive and semiquantitative for identifying transgenic plants and progeny from Biolistic? and protoplast transformations is described. Leaf sections of maize and wheat plants are placed on an indicator medium containing
chlorophenol red and the selection agent. Changes in the color of the medium from red to yellow resulting from altered pH
indicate transformed plants within 2-5 d. The method is particularly suited to use with phosphinothricin and could be used
with other suitable selectable markers. |
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Keywords: | Zea mays Triticum transgenic Bt phosphinothricin indicator |
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