肺结核患者血清IL-6、IL-12、IL-23、TNF-α、IFN-γ表达水平的临床意义分析

目的探讨肺结核患者血清中巨噬细胞相关细胞因子IL-6、IL-12、IL-23、TNF-α、IFN-γ的变化及其临床意义。方法采用酶联免疫吸附法(ELISA)检测30例活动性肺结核患者(患者);30例结核分枝杆菌感染者(感染者);30例健康人血清中的IL-6、IL-12、IL-23、TNF-α、IFN-γ分泌水平。结果 IL-6在健康人、患者者和感染组血清中的分泌水平分别为:0.43±0.03 pg/ml,0.86±0.04 pg/ml,0.46±0.02 pg/ml;IL-12在健康人、患者者和感染组血清中水平分别为:9.43±0.22 pg/ml,14.96±0.58 pg/ml,9.58±0.20 pg/ml;IL-23在健康人、患者者和感染组血清中水平分别为:269.58±28.58pg/ml,336.58±30.61 pg/ml;332.03±25.71 pg/ml;IFN-γ在健康人、患者者和感染组血清中水平分别为:90.80±2.05 pg/ml,116.15±4.96 pg/ml,114.29±3.16 pg/ml;TNF-α在健康人、患者者和感染组血清中水平分别为:13.87±3.28 pg/ml,14.06±2.14 pg/ml,13.43±3.12 pg/ml。统计分析发现:IL-6、IL-12、IL-23、IFN-γ在患者血清中的分泌水平显著高于健康对照组(p<0.05);IL-23与INF-γ在感染者血清中的分泌水平显著高于健康对照组(p<0.05);IL-6与IL-12在患者血清中的分泌水平显著感染者组(p<0.05);其余细胞因子在3类人群血清中分泌水平差别无统计学意义。结论血清中巨噬细胞相关细胞因子IL-6、IL-12、IL-23、IFN-γ在肺结核发病中具有重要意义,具有成为肺结核临床诊断依据可能。

CLINICAL SIGNIFICANCE OF DETECTING CYTOKINES SECRETE BY MACROPHAGE IN SERUM OF PATIENTS WITH PULMONARY TUBERCULOSIS

Objective To explore the Clinical significance of detecting cytokines （ IL - 6 ,IL - 12, IL - 23 ,TNF-α、IFN-γ） secrete by macrophage in serum of patients. Method ELISA method was used to measure the level of five cytokines （ IL - 6, IL - 12, IL -23, TNF-α、IFN-γ） expression of the different groups, including 30 cases of patients with active pulmonary tuberculo- sis, 30 cases of people with latent Mycobacterium Tuberculosis, 30 cases of healthy controls. Result The levels of IL - 6 in healthy control, pulmonary tuberculosis group and latent Mycobacterium tuberculosis group respectively was 0. 43 ± 0. 03 pg/ml, 0.86 ± 0. 04 pg/ml, 0. 46 ± 0. 02 pg/ml; The levels of IL - 12 in healthy control, pulmonary tuberculosis group and latent Mycobacterium tubercu- losis group respectively was 9.43 ±0. 22 pg/ml, 14. 96 ±0. 58 pg/ml, 9.58 ±0. 20 pg/ml; The levels of IL -23 in healthy control, pulmonary tuberculosis group and latent Mycobacterium tuberculosis group respectively was 269.58 ± 28.58 pg/ml, 336.58 ± 30. 61 pg/ml, 332.03± 25.71 pg/ml; The levels of IFN -α, in healthy control, pulmonary tuberculosis group and latent Mycobacterium tu- berculosis group respectively was 90. 80 ± 2.05 pg/ml, 116. 15 ± 4. 96 pg/ml, 114. 29 ± 3.16 pg/ml; The levels of TNF -α in healthy control, pulmonary tuberculosis group and latent Mycobacterium tuberculosis group respectively was 13.87 ± 3.28 pg/ml, 14.06 ± 2. 14 pg/ml, 13.43 ± 3.12 pg/ml. Statistical analysis found that Levels of IL - 6, IL - 12, IL - 23 and IFN -γ in pulmonary tuberculosis group were significantly higher than those in healthy control group （p 〈 0. 05 ）, IL -23 ,IFN-γ in latent Mycobacterium tuberculosis group were significantly higher than those in healthy control group. Compared with the latent Mycobacterium Tuberculosis group, the levels of serum IL -6 ,IL - 12 in the active pulmonary tuberculosis group were significantly higher （p 〈 0. 05 ）. The other cytokines levels in active pulmonary tuberculosis group, latent Mycob