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青霉菌发酵液对大豆幼苗生长及生理特性的影响
引用本文:陶波,张健,韩玉军,王小琴,乔禹欣,祖永平.青霉菌发酵液对大豆幼苗生长及生理特性的影响[J].大豆科学,2017,36(3).
作者姓名:陶波  张健  韩玉军  王小琴  乔禹欣  祖永平
作者单位:东北农业大学农学院,黑龙江哈尔滨,150030
基金项目:转基因生物新品种培育重大专项
摘    要:采用室内生理检测及生物化学方法,研究了青霉菌发酵液对大豆幼苗生长及生理特性的影响。结果表明:用一定浓度的青霉菌发酵液对大豆浸种和茎叶处理,大豆幼苗的生长呈明显上升趋势,处理后大豆的株高、根长、株鲜重和根鲜重均明显得到了提高,且生长状态好于对照,不同浓度处理间具有明显差异,在发酵液浓度为5 g·L-1时,效果最佳。尤其是对根鲜重的影响,促进率最高可达56.21%,同时增强了幼苗根系活力,最大可以提高57.82%,浸种和茎叶处理后,分别提高了叶绿素含量41.25%和15.40%;超氧化物歧化酶活性12.20%和11.70%;过氧化物酶31.33%和29.95%;过氧化氢酶活性42.8%和20.0%,这为发酵液在大豆田推广应用、提高大豆产量提供了重要理论依据。

关 键 词:青霉菌发酵液  大豆  根系活力  超氧化物歧化酶  过氧化物酶  过氧化氢酶

Effect of Penicillium Fermented Liquid on Seedling Growth and Physiological Characteristics of Soybean
TAO Bo,ZHANG Jian,HAN Yu-jun,WANG Xiao-qin,QIAO Yu-xin,ZU Yong-ping.Effect of Penicillium Fermented Liquid on Seedling Growth and Physiological Characteristics of Soybean[J].Soybean Science,2017,36(3).
Authors:TAO Bo  ZHANG Jian  HAN Yu-jun  WANG Xiao-qin  QIAO Yu-xin  ZU Yong-ping
Abstract:In this paper,indoor physical test and biochemical methods were used to research the infuence of penicillium fermented liquid to soybean seedling growth and physiological characteristics.The results showed that after the soybean seed and foliage treated with the certain concentration fermented liquid treatment,soybean seedling growth was rising significantly,the plant height,root length,fresh weight and root fresh weight of soybean were significantly improved,and the growth status was better than control,and different concentrations had obvious differences.When the fermented liquid concentration get 5 g·L-1,the effect was the best.Especially the root fresh weight,promoted rate reached 56.21%,enhanced seedling root activity at the same time,the maximum could increase by 57.82%.After seed and foliage treatment,the increment of the chlorophyll content were 41.25% and 15.40% respectively,superoxide dismutase (SOD) were 12.2% and 1 1.70%,peroxidase were 31.33% and 29.95%,hydrogen peroxide enzyme activity were 42.8% and 20.0%.It provides important theoretical basis for fermented liquid application in soybean field and improving soybean yield.
Keywords:Penicillium fermented liquid  Soybean  Root activity  Superoxide dismutase (SOD)  Peroxidase (POD)  Catalase (CAT)
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