| 马铃薯醇溶蛋白电泳分析方法的建立 |
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| 引用本文： | 芶琳,李文博,倪苏,程浩然.马铃薯醇溶蛋白电泳分析方法的建立[J].安徽农业科学,2011,39(1):71-72. |
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| 作者姓名： | 芶琳 李文博 倪苏 程浩然 |
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| 作者单位： | 四川农业大学生命科学与理学院,四川雅安,625014;四川农业大学农学院,四川雅安,625014 |
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| 基金项目： | 四川省科技厅马铃薯优质高产关键技术研究与产业化示范项目(05NG001-021-2); 植物生产类国家级人才培养模式创新试验区项目(00108902) |
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| 摘 要： | 目的]探索并建立马铃薯醇溶蛋白酸性聚丙烯酰胺凝胶电泳（A-PAGE）方法和谱带分析技术。方法]用25%2-氯乙醇提取马铃薯醇溶蛋白,借助酸性聚丙烯酰胺凝胶电泳方法对马铃薯醇溶蛋白进行分析,电泳采用浓度12.4%聚丙烯酰胺凝胶,考马斯亮蓝染色。结果]不同品种都显示出各自特有的醇溶蛋白带谱组合,且清晰可辨。结论]酸性凝胶电泳技术可用于马铃薯醇溶蛋白分析,具有快速、分辨率高、重复性好等特点,可为马铃薯品种（系）选育提供一种十分有效的方法。
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| 关 键 词： | 马铃薯 醇溶蛋白 电泳 |
Establishment of Method of Potato (Solanum tuberosum L.) Alcohol-soluble Protein Electrophoresis Analysis |
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| GOU Lin et al.Establishment of Method of Potato (Solanum tuberosum L.) Alcohol-soluble Protein Electrophoresis Analysis[J].Journal of Anhui Agricultural Sciences,2011,39(1):71-72. |
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| Authors: | GOU Lin |
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| Affiliation: | GOU Lin et al (College of Biology and Science,Sichuan Agriculture University,Yaan,Sichuan 625014) |
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| Abstract: | Objective] The research aimed to explore and establish a method of potato alcohol-soluble protein electrophoresis. Method] Potato alcohol-soluble protein was extracted with 25% 2-chloro-ethanol with the help of A-PAGE to analyze potato alcohol-soluble protein. The concentration of gel was 12.4% and it was dyed with coomassie brilliant blue. Result] All varieties had their unique combination of alcohol-soluble protein band spectral. Conclusion] A-PAGE could be used for potato alcohol-soluble protein anal... |
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| Keywords: | Potato Alcohol-soluble protein Electrophoresis |
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