Two-step chromatographic procedure for the preparation of hen egg white ovotransferrin |
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Authors: | Thomas Croguennec Françoise Nau Stéphane Pezennec Michel Piot Gérard Brulé |
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Affiliation: | (1) UMR INRA-ENSA, Technologie des Ovoproduits, 65, rue de St Brieuc, 35042 Rennes Cedex, France e-mail: nau@agrorennes.educagri.fr, FR |
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Abstract: | A two-step chromatographic procedure for the preparation of hen egg white ovotransferrin by using an anion exchanger is described. The method consists of a preliminary step of frontal chromatography, also called displacement chromatography, on a Q Sepharose Fast Flow column. This simple and powerful chromatographic step enables the pre-purification of ovotransferrin that is further purified by a second conventional preparative chromatography performed on the same Q Sepharose FF column. The ovotransferrin obtained is found to be homogeneous in electrophoresis and analytical chromatography. Its purity rate is calculated at 97.5% and its amino acid composition is close to that obtained from the nucleotide sequence of ovotransferrin. Moreover, ovotransferrin is iron-free, thus suitable for studies in relation to its metal-binding activity. Received: 31 March 2000 / Revised version: 29 May 2000 |
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Keywords: | Ovotransferrin Chromatography Purification Anion exchanger |
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