p57KIP2、cyclin D1和cyclin E在宫颈鳞癌中的表达及意义

目的探讨p57^KIP2、cyclin D1及cyclin E蛋白在宫颈癌发生、发展中的作用。方法用免疫组织化学SP法检测100例宫颈鳞癌、60例宫颈上皮内瘤变和30例正常宫颈鳞状上皮组织中p57^KIP2、cyclin D1和cyclin E蛋白的表达情况。结果cyclin D1、cyclin E蛋白在宫颈SCC与NE、CIN与NE组织中阳性表达率之间比较、cyclin E蛋白在宫颈SCC与CIN组织中阳性表达率之间比较，差异均有显著性（P〈0．01）；cyclin D1与cyclin E之间的表达呈正相关（P〈0．01）；三者表达均与组织学分级、淋巴结转移、患者年龄无关（P〉0．05）。结论p57^KIP2、cyclin D1及cyclin E蛋白共同参与了宫颈癌的发生发展。cyclin D1和cyclin E蛋白高表达可能是宫颈组织恶变的重要生物学标志，cyclin E异常表达是宫颈癌发生的早期事件。

Expression of p57KIP2, cyclin D1, and cyclin E and Their Clinical Significances in Squamous Cell Carcinoma of Uterine Cervix

Objective To discuss the effects of p57KIP2,cyclin D1,and cyclin E on the genesis and progression of carcinoma of the uterine cervix. Methods The expression of p57KIP2, cyclin D1,and cyclin E proteins in 100 cases of squamous cell carcinoma of the uterine cervix,60 cases of cervical intraepithelial neoplasia(CIN),and 30 cases of normal cervical squamous epithelium(NE) was detected respectively by immunohistochemical SP methods.Results The expression of cyclin D1,and cyclin E between SCC and NE,and between CIN and NE,and the expressionof cyclin E between CIN and NE had significant difference(P<0.01).In SCC, all the positive expression of p57KIP2,cyclin D1 and cyclin E were no correlation with lymph node metastasis,and histological grade,and age(P<0.01).There was positive relationship between expression of cyclin D1 and cyclin E(P<0.01).Conclusion p57KIP2,cyclin D1,and cyclin E might be useful in the development and progression of carcinoma of the uterine cervix.The over-expression of cyclin D1 and/or cyclin E proteins in SCC suggest that they might be important biological marker for malignant transformation in uterine cervix tissueand,and the abnormal expression of cyclin E were early event of carcinoma of the uterine cervix.