| 应用依赖解旋酶DNA恒温扩增技术检测副溶血性弧菌 |
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| 引用本文： | 石琰璟,王建广,房保海,张健,祝素珍,刘云国,姜英辉,雷质文,杨大伟.应用依赖解旋酶DNA恒温扩增技术检测副溶血性弧菌[J].青岛科技大学学报,2011,32(1). |
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| 作者姓名： | 石琰璟 王建广 房保海 张健 祝素珍 刘云国 姜英辉 雷质文 杨大伟 |
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| 作者单位： | 石琰璟,SHI Yan-jing(青岛科技大学,化工学院,山东,青岛,266042);王建广,WANG Jian-guang(青岛科技大学,化工学院,山东,青岛,266042;山东出入境检验检疫局,山东,青岛,266002);房保海,张健,祝素珍,刘云国,姜英辉,雷质文,杨大伟,FANG Bao-hai,ZHANG Jian,ZHU Su-zhen,LIU Yun-guo,JIANG Ying-hui,LEI Zhi-wen,YANG Da-wei(山东出入境检验检疫局,山东,青岛,266002) |
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| 基金项目： | 国家质检总局科研项目，山东出入境检验检疫局科研项目 |
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| 摘 要： | 以副溶血性弧菌的tlh基因为目的片段设计特异性引物,成功建立了恒温条件下快速检测副溶血性弧菌的依赖解旋酶DNA恒温扩增技术(HDA)的检测法,进行了特异性和灵敏度实验,并与普通PCR方法进行了比较。该HDA检测方法最低检测限为19.9ng·mL-1,与普通PCR方法相当。副溶血性弧菌的HDA检测方法具有普通PCR的特异、灵敏等特点,并且对仪器要求更低,用普通水浴槽即可进行反应。
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| 关 键 词： | 副溶血性弧菌 检测 依赖解旋酶DNA恒温扩增技术 |
Detection of Vibrio Parahaemolyticus by Helicase-dependent Isothermal DNA Amplification Technology |
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| SHI Yan-jing,WANG Jian-guang,FANG Bao-hai,ZHANG Jian,ZHU Su-zhen,LIU Yun-guo,JIANG Ying-hui,LEI Zhi-wen,YANG Da-wei.Detection of Vibrio Parahaemolyticus by Helicase-dependent Isothermal DNA Amplification Technology[J].Journal of Qingdao University of Science and Technology:Natutral Science Edition,2011,32(1). |
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| Authors: | SHI Yan-jing WANG Jian-guang FANG Bao-hai ZHANG Jian ZHU Su-zhen LIU Yun-guo JIANG Ying-hui LEI Zhi-wen YANG Da-wei |
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| Abstract: | A new rapid method to detect Vibrio parahaemolyticus samples has been established on the basis of Helicase-dependent isothermal DNA amplification(HDA).A highly specific set of primers were synthesized to target the tlh gene of Vibrio parahaemolyticus.Specificity and sensitivity were tested by comparing with PCR method.The sensitivity was 19.9 ng·mL-1 which was similar to the result of PCR method.Detecting Vibrio parahaemolyticus with HDA is specific and sensitive as well as PCR method and has lower instrumental requirement. |
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| Keywords: | Vibrio parahaemolyticus detection helicase-dependent isothermal DNA amplification |
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