MRSA分子流行病学研究 FREE

目的 采用脉冲场凝胶电泳（PFGE）分型及多重聚合酶链反应（PCR）SCCmec分型技术对耐甲氧西林金黄色葡萄球菌（MRSA）进行基因分型，以了解医院感染MRSA的流行情况。方法 收集天津市南开医院2007年1月-2008年12月住院患者送检标本分离的MRSA 40株，进行耐药谱分析、PFGE分型和SCCmec分型。结果40株菌耐药谱高度相似，可被分为A-E 5个PFGE型，其中A型21株，B型8株，C型4株，D型6株，E型1株；SCCmec分型：Ⅰ型1株，Ⅲ型33株，Ⅳ型1株，V型5株。通过对医院内菌株发生的时间和空间分析，明确了感染的发生和传播情况，即在2007年1月-2008年12月存在MRSA散发感染。结论 PFGE可有效鉴定菌株间的亲缘关系，SCCmec分型对是否为医院感染菌株有提示作用，两种分型方法相结合可更好地鉴定流行菌株的特征，为医院感染的监控提供依据。

Molecular epidemiological study of methicillin resistant Staphylococcus aureus

Objective To analyze the epidemiological characteristics of methicillin resistant Staphylococcus aureus （MRSA） in nosocomial infection with pulsed field gel electrophoresis （PFGE） and PCR SCCmec typing. Methods Forty strains of MRSA collected from Tianjin Nankai Hospital between January, 2007 and December, 2008 were performed antimicrobial susceptibility analysis, PFGE and SCCmec genotyping. Results Antimicrobial susceptibility patterns of 40 strains of MRSA were highly similar, isolates were classified into A-E five types by PFGE typing, type A, B, C, D, E were 21, 8, 4, 6, I strains respectively; SCCmec typing showed that I strain was SCCmec I , 33 were SCCmec III, 1 was SCCmec IV ,and 5 were SCCmecV. Sporadic infection of MRSA occurred in the hospital during the studied period. Conclusion Epidemiological analysis indicates that PFGE typing can identify the genetic relationship between isolates, SCCmec typing can suggest whether MRSA isolates are hospital-acquired. Combination of two genotyping methods can show characteristics of epidemic strains, and provide reference for nosocomial infection surveillance.