人前磨牙牙髓牙本质复合体层粘连蛋白的免疫组织化学研究

目的　研究层粘连蛋白在牙髓牙本质复合体内的表达 ,探讨其分布与功能的关系。　方法　收集不同年龄新鲜健康人前磨牙 6 0颗 ,固定 ,脱钙 ,石蜡包埋 ,层粘连蛋白免疫组织化学染色。　结果　层粘连蛋白免疫反应性 ( IR)普遍存在于牙髓、前期牙本质和成熟牙本质小管与成牙本质细胞突起之间 ,以牙髓的神经与血管周围最为丰富 ,冠髓较根髓密集。　结论　层粘连蛋白 IR不仅见于牙髓与前期牙本质 ,而且也见于成熟牙本质 ,它对支持、固定牙髓牙本质复合体各结构的正常位置、形态可能发挥了重要作用

IMMUNOHISTOCHEMICAL STAINING OF LAMININ IN DENTAL PULP-DENTIN COMPLEX OF HUMAN PREMOLAR

Objective The expression of laminin was detected in dental pulp dentin complex of human premolar to find out the relationship between disribution and function of laminin. Methods Sixty human fresh and healthy human premolars were collected,fixed,decalcified,embedded in paraffin and cut into slices,then the slices were treated by immunohistochemical reaction of laminin. Results Laminin immunoreactivity was presented in dental pulp and predentin,and also in space between the wall of dentinal tubules and processes of odontoblast in mature dentin.It was most abundant around blood vessels and nerves in dental pulp,moreover,more abundant in crown pulp than in root pulp.Conclusions Laminin immunoreactivity usually appeared in dental pulp and predentin and also in mature dentin,suggesting that it may play an important role in maintaining normal form and position of pulp cells and other structures in dental pulp dentin complex.