输精管结扎家兔自身免疫反应的实验研究

33只成年雄兔行双侧输精管结扎手术后,进行连续12个月的免疫学观察。7只同龄雄兔作为空白对照。结果表明,间接血凝试验69.7%结扎组家兔检测出抗精子抗体,滴度范围为1:5～1:1280;间接免疫荧光测定有90.9%的实验组家兔测出抗精子抗体。白细胞粘附抑制实验呈阳性反应者占实验组家兔的43.5%,与对照组比较差异显著。应用PEG光密度和抗补体法测定CIC均里阴性。输精管结扎后第3个月附睾肿胀者占45%,第5—7个月达70%,之后逐渐消退。而与此同时抗精子抗体阳性检出率明显增高,故可推断精子抗原主要经附睾入血,作用于免疫系统导致体液与细胞免疫反应。结扎组家兔胸腺、脾、淋巴结呈明显的增生现象支持这一结论。

Experimental studies of autoimmune response to sperm antigens in vaseetomized rabbits

Thirty-three rabbits were bilaterally vasectomized and observed for 1-12 months with 7 normal male rabbits control. It was found that (1) 67.9% of the vase ctomized rabbits revealed antibodies to sperm antigens with a titre ranging from 1:5 to 1:1280 as detected by indirect hemagglutination test; (2) 90.9% of the vasectomized rabbits showed antibodies to sperm by indirect immunofluorescence; (3) 43.5% of the vasectomized rabbits showed positive leucocyte adhesion inhibition test; (4) while pathomorphological findings in the section of immune organs of the vasectomized rabbits showed that the lymphatic tissues were proliferative, macrophages were active. Circulatory immune complex was not detected in any of the 33 vasectomized rabbits. Epididymides were swollen in 70% of the experimental animals in 5-7 months after vasectomy, subsidence of epididymal swelling occurred usually with the increase of antibody titres against sperm antigen.