| 熟制鲍鱼残留细菌的分离及16S rDNA鉴定 |
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| 作者姓名： | 郑瑞生 陈凉凉 肖 熙 |
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| 作者单位： | 泉州师范学院化学与生命科学学院,福建泉州 362002;福建农林大学食品科学学院,福建福州 350002;泉州师范学院化学与生命科学学院,福建泉州,362002;福建农林大学食品科学学院,福建福州,350002 |
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| 基金项目： | 福建省大学生创新创业训练计划;福建省教育厅科技项目;泉州市优秀人才培养专项;泉州市科技计划重点项目 |
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| 摘 要： | 为控制熟制鲍鱼残留的细菌污染，采用平板划线分离技术对烘制加工后鲍鱼中残留的主要菌群进行分离，并通过菌落形态观察、革兰氏染色分析和16S rDNA 序列比对等方法对其进行鉴定. 结果表明，残留在烘制加工后鲍鱼的主要菌群有希瓦氏菌属、不动杆菌属、库特氏杆菌属、海洋类香菌、蜡样芽孢杆菌、肠杆菌属、彭氏变形杆菌等.
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| 关 键 词： | 烘制鲍鱼 残留细菌 16S rDNA 鉴定 |
Separation and 16S rDNA Identification of Main Residual Bacteria from Baked Abalone |
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| Authors: | ZHENG Rui-sheng CHEN Liang-liang and XIAO Xi |
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| Affiliation: | College of Chemistry and Life Sciences, Quanzhou Normal University
College of Food Science, Fujian Agriculture and Forestry University;College of Chemistry and Life Sciences, Quanzhou Normal University;College of Food Science, Fujian Agriculture and Forestry University |
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| Abstract: | In order to control the main residual bacterial population of baked abalone, the streak plate separating technology was used to isolate the primary residual bacterial floras. And the main bacteria were identified according to the colony morphology observation, gram staining, and 16S rDNA sequence alignment. The result indicated that the primary residual bacteria in baked abalone were Shewanella sp., Acinetobacter sp., Kurthia sp., Myroides pelagicus, Bacillus cereus, Enterobacter sp., Proteus penner, and so on. |
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| Keywords: | baked abalone residual bacteria 16S rDNA identification |
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