从我国成人手足口病患者疱液中首次分离出肠道病毒71型

本文报道了从我国手足口病(HFMD)患者疱液中肠道病毒71(E71)型H株的分离和鉴定。该株病毒可在原代人胚肺(HEL)细胞、MA104细胞、BSC细胞中生长繁殖,导致典型的肠道病毒CPE出现。将H株接种乳鼠后出现肢体麻痹、第6天开始死亡。电镜下可见感染H株的BSC细胞胞浆中出现大量的结晶状排列的成熟病毒颗粒,直径约25nm。患者双份血清中有对H株4倍增高的中和抗体存在。采用100抗体单位的抗Cox A5、7、9、16、E70、E71抗体和50抗体单位的LBM组合血清A-H以及抗E71BrCr株MeAb P27对H株进行中和试验时,H株可被抗E71血清和MeAb P27所中和。抗E71抗体对H株的最低有效中和作用为1.6抗体单位,MeAb P27对H株的有效中和作用是64抗体单位。其它血清则无此中和作用。然而,在鉴定过程中发现,高滴度的抗Cox A16抗体(200抗体单位以上)也显出有中和H株的作用,提示我们所分离的H株含有与Cox A16的型间共同抗原。

Isolation of Enterovirus Type 71 from the Vesicle Fluid of an Adult Patient with Hand-footmouth Disease in China

Isolation and characterization of a H isolate of enterovirus type 71 (E71) from the vesicle fluid of hand-foot-mouth disease have been reported in present study.The H isolate can grow at primary human embryo lung cells, MA104 cells, and BSC cells and results in a typical enterovirus cytopathic effect. 1-day-old mice inoculated ip with H isolate showed limb paralysis and died at 6th day postinfection. Numerous mature virus particles with a diameter of 25 nm in crystal arrays in the cytoplasm have been visualized under electron microscope. The paired sera of the patient have been tested and showed a 4-fold increase of neutralizing antibody to H isolate.Using 100 antibody units of anti-Cox A5, 7, 9, 16, E70, E71, and 50 antibody units of enterovirus LBM typing pools A-H as well as McAb p27 of E71 BrCr strain, H isolate could be only neutralized by both anti-E71 polyclone and monoclone antibodies. The minimal effective antibody units to H isolate are required respectively 1.6 for anti-E71 polyclone antibody and 64 for McAb p27, Furthermore, high titers of anti-Cox A16 have also showed some cross reaction to H isolate by neutralization test,suggesting that H isolate shares a common antigen with Cox A16.