p16、p21在涎腺多形性腺瘤及恶性多形性腺瘤中的表达

目的:研究涎腺多形性腺瘤及恶性多形性腺瘤中抑癌基因p16、癌基因rasp21的表达,探讨基因在涎腺多形性腺瘤及恶性多形性腺瘤发生发展中的作用。方法:应用免疫组化SABC法检测正常涎腺组织、多形性腺瘤及恶性多形性腺瘤中p16、p21的表达。结果:(1)p16表达:正常组阳性表达率为100%,良性组为95%,恶性组为82.5%。正常组与恶性组、良性组与恶性组相比存在显著性差异(P<0.05),正常组与良性组之间无统计学差别。(2)p21表达:正常组阳性表达率为0%,良性组为65%,恶性组为72.5%。正常组与良性组或恶性组相比均具有显著性差异(P<0.01),但良性组与恶性组相比无显著差别。结论: 1.p16基因变异在涎腺多形性腺瘤及恶性多形性腺瘤的发生发展中起一定作用;2.ras基因产物p21过表达可能对涎腺多形性腺瘤及恶性多形性腺瘤的早期发生起重要作用。

The Expression of p16,p21 in Pleomorphic Adenoma and Malignant Pleomorphic Adenoma of Salivary Gland

Objective: To investigate the expression of p16?p21 in the tissues of pleomorphic adenoma (PA) and malignant pleomorphic adenoma (MPA) and to reveal their roles in the occurrence and development of PA and MPA. Methods: We studied the expression of p16?p21 in 10 samples of normal salivary gland tissues(NM),60 samples of PA, 40 samples of MPA by SABC immunohistochemichal technique. Results: 1.p16 expression: The positive expression rate was 100% in NM, 95% in PA and 82.5% in MPA.There were significant statistical differences (P<0.05) between NM and MPA,PA and MPA,but not between NM and PA. 2.p21expression:The positive expression rate was 0% in NM,65% in PA and 72.5% in MPA.There were significant statistical differences (P<0.01) between NM and PA, NM and MPA, but not between PA and MPA. Conclusion: 1.The results indicate that p16 gene aberration may be involved in the development of PA and MPA; 2.The overexpression of p21 may play an important role in the early stage of the tumorigenesis.