| 转移生长因子β1在牛眼小梁网的表达 |
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| 引用本文： | 袁莉娅 魏厚仁. 转移生长因子β1在牛眼小梁网的表达[J]. 中华眼科杂志, 1997, 33(4): 301-303 |
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| 作者姓名： | 袁莉娅 魏厚仁 |
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| 作者单位： | 同济医科大学附属协和医院眼科 |
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| 摘 要： | 目的研究转移生长因子β１（ｔｒａｎｓｆｏｒｍｉｎｇｇｒｏｗｔｈｆａｃｔｏｒβ１，ＴＧＦ－β１）与开角型青光眼的关系。方法用异硫氰酸胍法提取２４只新生牛眼小梁网的总ＲＮＡ，将ＴＧＦ－β３３质粒导入大肠杆菌ＨＢ１０１中充分扩增、ＢａｍＨＩ酶切后，片段以α－３２－Ｐ－ｄＡＴＰ标记成ｃＤＮＡ（ｃｏｍｐｌｅｍｅｎｔａｒｙＤＮＡ）探针，ＲＮＡ斑点杂交，放射自显影法观察和测定牛眼小梁网的ＴＧＦ－β１ｍＲＮＡ的表达。结果牛眼小梁网中有ＴＧＦ－β１ｍＲＮＡ的表达。结论房水中的部分ＴＧＦ－β１由小梁细胞分泌。此结果可以作为利用分子生物学手段研究ＴＧＦ－β１ｍＲＮＡ的异常合成、活化、清除及与开角型青光眼病理改变间关系的基础。
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| 关 键 词： | 小梁网 转移生长因子 动物.实验青光眼.开角型 |
Expression of messenger RNA for transforming growth factor-beta 1 in bovine trabecular meshwork] |
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| Yuan Liya,Wei Houren. Expression of messenger RNA for transforming growth factor-beta 1 in bovine trabecular meshwork][J]. Chinese Journal of Ophthalmology, 1997, 33(4): 301-303 |
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| Authors: | Yuan Liya Wei Houren |
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| Affiliation: | Department of Ophthalmology, Union Hospital of Tongji Medical University, Wuhan. |
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| Abstract: | OBJECTIVE: To investigate the relationship between transforming growth factor-beta 1 (TGF-beta 1) and primary open-angle glaucoma. METHODS: Total RNA of 24 new born bovine trabecular tissue was extracted by guanidine isothiocyanate method. The TGF-beta 33 plasmid was brought into colibacillus HB101 and amplified. After Bam HI enzyme degradation and label with alpha-32P-dATP, the RNA was hybridized with the complementary DNA (cDNA) probe and examined by autoradiography to determine the expression of messenger RNA (mRNA) for TGF-beta 1. RESULTS: The presence of mRNA for TGF-beta 1 in bovine trabecular meshwork was confirmed. CONCLUSION: The TGF-beta 1 present in normal aqueous humor at least partly is derived from the trabecular meshwork. The result offers a basis for understanding the relationship between abnormal synthesis, activation and clearance of TGF-beta 1 and the pathogenesis of primary open-angle glaucoma in molecular biology. |
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| Keywords: | Trabecular meshwork Transforming growth factor Animal laboratory Glaucoma open angle |
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