| 除草剂莎稗磷多克隆抗体的制备 |
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| 引用本文： | 高爱华,谈超,张燕,王硕.除草剂莎稗磷多克隆抗体的制备[J].食品与机械,2012,28(3):87-89. |
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| 作者姓名： | 高爱华 谈超 张燕 王硕 |
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| 作者单位： | 食品营养与安全省部共建教育部重点实验室,天津300457;天津科技大学食品工程与生物技术学院,天津300457 |
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| 基金项目： | “十一五”国家科技支撑计划 |
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| 摘 要： | 根据莎稗磷的化学结构特征,设计合成莎稗磷半抗原,采用活化酯法与牛血清白蛋白偶联,免疫新西兰大耳白兔获得抗莎稗磷多克隆抗血清用于免疫分析试验。抗血清效价高达1∶160 000,100μg/L莎稗磷的抑制率达到了59.76%。抗体经Protein A-Sepharose 4B亲和层析柱纯化后,50μg/L莎稗磷的抑制率提高到92%,其它有机磷农药及结构类似化合物与抗体的交叉反应率均小于0.01%,表明了所制备莎稗磷抗体的高特异性。
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| 关 键 词： | 莎稗磷 免疫分析 多克隆抗体 高特异性 |
Preparation of polyclonal antibody for herbicide anilofos |
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| GAO Ai-hua
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TAN Chao
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ZHANG Yan
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WANG Shuo.Preparation of polyclonal antibody for herbicide anilofos[J].Food and Machinery,2012,28(3):87-89. |
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| Authors: | GAO Ai-hua TAN Chao ZHANG Yan WANG Shuo |
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| Affiliation: | 1,2 (1.Key Laboratory of Food Nutrition and Safety,Ministry of Education,Tianjin 300457,China;2.Faculty ofFood Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China) |
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| Abstract: | An immunosorbent assay for anilofos was developed by using rabbit polyclonal antibodies against a hapten-protein conjugate of anilofos-bovine serum albumin(BSA).The anti-anilofos polyclonal antiserum with a titer of 1∶160 000 was obtained which had an inhibition of 59.76% to anilofos(100 μg/L).The inhibition had reached 92% to anilofos of 50 μg/L after the antiserum purified by Protein A-Sepharose 4B.No cross-reactivity with other structurally similar compounds showed high specificity. |
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| Keywords: | anilofos immunosorbent assay polyclonal antibodies high specificity |
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