| 芝麻过敏原PCR检测方法 |
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| 引用本文： | 张文举,许庆金,邓志瑞,陈沁. 芝麻过敏原PCR检测方法[J]. 食品与机械, 2012, 28(2): 52-55,65 |
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| 作者姓名： | 张文举 许庆金 邓志瑞 陈沁 |
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| 作者单位： | 上海大学生命科学学院,上海,200444 |
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| 摘 要： | 芝麻是重要的食品过敏原之一,微小剂量即可引起严重的过敏反应。针对芝麻过敏蛋白-2S白蛋白的基因序列设计PCR扩增引物,建立并优化芝麻过敏原检测的普通聚合酶链式反应(PCR)和实时荧光定量聚合酶链式反应(Real-time PCR)方法。结果表明,两种方法的最低检测限分别为0.1,0.01ng DNA,该方法特异性良好,成本较低,具有一定的应用价值。
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| 关 键 词： | 芝麻 过敏原 聚合酶链式反应 实时荧光定量聚合酶链式反应 2S白蛋白 |
Establishment of PCR method for detecting sesame allergen |
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| ZHANG Wen-ju , XU Qing-jin , DENG Zhi-rui , CHEN Qin. Establishment of PCR method for detecting sesame allergen[J]. Food and Machinery, 2012, 28(2): 52-55,65 |
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| Authors: | ZHANG Wen-ju XU Qing-jin DENG Zhi-rui CHEN Qin |
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| Affiliation: | (School of Life Sciences,Shanghai University,Shanghai 200444,China) |
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| Abstract: | Sesame is an important food allergen;low-level doses can cause severe allergic reactions.In this paper,PCR primers were designed based on sesame allergy protein(2S albumin) gene sequence,and conventional PCR and Real-time PCR were established and optimized.The lowest DNA content that can be detected for conventional PCR was 0.1 ng and 0.01 ng for real-time PCR,respectively.The specificity of the methods is good and can be widely used. |
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| Keywords: | sesame allergen PCR Real-time PCR 2S albumin |
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