| 紫外分光光度法测定青蒿素的含量 |
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| 引用本文： | 李春莉,王莎莉,王亚平,柯大智. 紫外分光光度法测定青蒿素的含量[J]. 重庆医科大学学报, 2007, 32(4): 413-415 |
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| 作者姓名： | 李春莉 王莎莉 王亚平 柯大智 |
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| 作者单位： | 重庆医科大学基础医学研究所,重庆,400016;重庆医科大学基础医学院生理教研室,重庆,400016;重庆医科大学基础医学院组胚教研室,重庆,400016;重庆医科大学附属第二医院老年病科,重庆,400010 |
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| 摘 要： | 目的:建立测定青蒿中青蒿素含量的紫外分光光度检测方法,为筛选收购优质青蒿提供快速检测的方法.方法:用紫外分光光度计对青蒿素标准溶液进行波长扫描,确定最适吸收波长,在最适吸收波长下建立青蒿素标准品的吸光度-浓度曲线.根据标准曲线测定青蒿样品中青蒿素的含量.结果:青蒿素的最适吸收波长为290.5nm,吸光度-浓度回归方程为:Y=0.043X 0.006,不同青蒿样品中青蒿素含量有差异.结论:紫外分光光度法测定青蒿中青蒿素成分含量操作简便、快捷、准确.
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| 关 键 词： | 青蒿素 紫外-可见分光光度法 含量测定 |
| 文章编号： | 0253-3626(2007)04-0413-03 |
| 修稿时间： | 2006-10-30 |
Determination of arteannuin element content by the ultraviolet spectrophotometric |
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| LI Chunli,et al. Determination of arteannuin element content by the ultraviolet spectrophotometric[J]. Journal of Chongqing Medical University, 2007, 32(4): 413-415 |
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| Authors: | LI Chunli et al |
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| Affiliation: | Institute for Basic Medical Science, Chongqing Medical University |
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| Abstract: | Objective:Establishing a simple useful method of the ultraviolet spectrophotometric to determine the arteannuin element content,providing the fast basis for screening and shopping high quality arteannuin.Method:Carrying on the wave length scanning with the ultraviolet spectrophotometer to arteannuin element standard solution, establishing the standard curve, according to standard curve determination sample content. Results:Arteannuin element most suitable absorption wave length is 290 nm, and the extinction-density regression equation is:Y=0.043X+0.006. The different arteannuin element sample content has the difference.Conclusion:The method of ultraviolet spectrophotometric determining arteannuin element content operation is quickly,simple and accurate. |
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| Keywords: | Arteannuin Ultraviolet-obvious spectrophotometric method Content determination |
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