奥扎格雷钠对实验性心肌缺血大鼠心肌组织NO及eNOS的影响

目的:观察奥扎格雷钠对实验性心肌缺血大鼠心肌组织NO及eNOS的影响。方法:将心电图正常的健康大鼠随机分成空白对照组(0.15mL生理盐水)、模型对照组(0.15mL生理盐水)、低剂量组(8g/kg奥扎格雷钠)、中剂量组(12g/kg奥扎格雷钠)、高剂量组(16g/kg奥扎格雷钠)和阳性对照组(81.0mg/kg复方丹参滴丸),连续灌胃给药7d,从第3d起除空白对照组外,其余各组均于给药后1h皮下注射异丙肾上腺素5mg/kg.d,连续5d,时间间隔为24h,正常对照组给予等量生理盐水皮下注射,末次注射异丙肾上腺素2h后取心肌组织测定NO、eNOS的活性。结果:与空白对照组相比,模型对照组大鼠的eNOS活力、总NOS活力明显提高,NO浓度明显降低(P<0.05);与模型对照组相比,低剂量组大鼠eNOS活力、总NOS活力降低,NO浓度提高(P>0.05),差异不显著,无统计学意义,而中剂量组及高剂量组、阳性对照组大鼠血清eNOS活力、总NOS活力明显减轻,NO浓度明显提高(P<0.05);中剂量组及高剂量组与阳性对照组比较,eNOS活力、总NOS活力降低程度,NO浓度提高程度相当。结论:奥扎格雷钠能有效地升高心肌组织NO浓度,抑制eNOS及总NOS活力系数,清除自由基,减轻心肌损伤。

Influence of ozagrel sodium on NO and eNOS in myocardial tissue of myocardial ischemia rat

Objective:To explore the influence of ozagrel sodium on NO and eNOS in myocardial tissue of myocardial ischemia rat.Methods:The electrocardiogram normal and health Wistar rats were randomly divided into control group(0.15 mL saline),model group(0.15 mL saline),low dose group(8 g/kg ozagrel sodium),middle dose group(12 g/kg ozagrel sodium),high dose group(16 g/kg ozagrel sodium) and positive control group(81.0 mg/kg nitroglycerin).They had continuous intragastric administration for 7 day.from the third day,in addition to the normal control group,the other groups had isoproterenol subcutaneous injection at 5 mg/kg·d 1 hour after administration for 5 days continuously,with interval time as 24 h.The normal control group received normal saline by subcutaneous injection.NO,eNOS of myocardial tissue were determined 2 hour after the injection of isoprenaline.Results:Compared with the control group,eNOS activity and total NOS activity were significantly increased,NO concentration was decreased significantly in model group(P<0.05);Compared with model group,eNOS activity and total NOS activity were decreased,and NO concentration was increased in low-dosage group(P>0.05).eNOS activity and total NOS activity were significantly decreased,and NO concentration was significantly increased in middle dose group,high dose group,and positive control group(P<0.05).Decrease in NOS activity and NOS activity was more significant in middle dose group and high dose group,and increase in NO concentration was not significant.Conclusions:Ozagrel sodium can effectively increase NO concentration in myocardial tissue,inhibit activity coefficient of eNOS and NOS,scavenge free radicals,and reduce myocardial injury.