体外诱导骨髓间充质干细胞分化为神经胶质样细胞的实验研究

目的：观察体外以不同方法诱导的骨髓间充质干细胞(Bone marrow mesenchymal stem cells，MSCs)分化为神经胶质样细胞，探讨MSCs作为组织工程周围神经种子细胞的可行性。方法：以大鼠MSCs为研究对象，分化学诱导组，共培养诱导组，未经诱导的MSCs作为对照组。通过体外动态观察细胞形态，免疫细胞化学，western blot，RT-PCR等手段对各组诱导后的细胞进行鉴定。结果：形态学观察显示两种诱导过程中的部分MSCs细胞都出现明显的神经胶质细胞的形态，共培养诱导组的MSCs在诱导后仍可继续传代培养，对照组细胞形态无变化。细胞免疫组织化学染色，western blot和RT-PCR结果显示两个诱导组细胞都能够表达神经干细胞标志性抗体(Nestin)、神经胶质纤维酸性蛋白抗体(GFAP)和S-100抗体。结论：我们推断MSCs很有可能成为TEPN的理想种子细胞来源。

Induced differentiation of bone marrow mesenchymal stem cell into glial -like cell in vitro

AIM:To observe induced differentiation of bone marrow mesenchymal stem cell (MSCs) into glial-like cells in vitro,and investigate the feasibility of MSCs being the seed cells of tissue engineered peripheral nerve (TEPN).METHODS:MSCs from adult rats were isolated,harvested and divided into chemical-inducing group,co-culture group and the undifferentiated control group.The morphology,immunocytochemistry, western blot and RT-PCR were used to identify cells type.RESULTS:Morphous of glial cells were observed in both inducing groups but not in control groups.MSCs in co-culture group remained acceleration and could be passaged yet after induction process.The expressing of protein and mRNA for nestin,glail fibrillary acidic protein (GFAP) and S-100 were similar to the morphological results.CONCLUSION:MSCs may be the ideal cells source of TEPN.