| 反相高效液相色谱法测定生化汤中阿魏酸及其血药浓度 |
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| 引用本文： | 宋金春,罗顺德,蔡鸿生,徐锋. 反相高效液相色谱法测定生化汤中阿魏酸及其血药浓度[J]. 中国医院药学杂志, 2001, 21(4): 213-215 |
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| 作者姓名： | 宋金春 罗顺德 蔡鸿生 徐锋 |
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| 作者单位： | 1. 武汉大学人民医院，湖北 武汉430060
2. 湖北医科大学药学系95级学生 |
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| 摘 要： | 目的建立一种测定生化汤中阿魏酸含量及兔口服生化汤后血清中游离阿魏酸浓度的方法。方法采用高效液相色谱法以甲醇-冰醋酸-水（30∶0.3∶69.7）为流动相，ZorbaxSB-C
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| 关 键 词： | 阿魏酸 生化汤 高效液相色谱法 血药浓度 中药 |
| 文章编号： | 1001-5213(2001)04-0213-03 |
| 修稿时间： | 2000-05-24 |
Determination of ferulicacid in Shenghua Tang and rabbit serum by RP-HPLC |
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| SONG Jin Chun,LUO Shun De,CAI Hong Sheng,et al. Determination of ferulicacid in Shenghua Tang and rabbit serum by RP-HPLC[J]. Chinese Journal of Hospital Pharmacy, 2001, 21(4): 213-215 |
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| Authors: | SONG Jin Chun LUO Shun De CAI Hong Sheng et al |
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| Abstract: | OBJECTIVE To establish a method for determinatin of ferulic acidin Shenghua Tang and rabbit serum. METHODS RP-HPLC was used. Mobile phase consisted of methanol-acetic acid-water (30∶0.3∶69.7) with UV detection at 323 nm,the analytical column was Zorbax SB-C18ODS 250 mm×4.6 mm, 5 μm. RESULTS The method was linear within the range of 0.22～3.52 μg·ml-1,r=0.9994, the within day RSD=0.33%(n=5). The mean recovery from serum was 98.3%. The extraction curves of ferulic acid in rabbit serum was linear over the range of 0.088～2.64 μg·ml-1,r=0.9989, the mean recovery from rabbit serum was 69.4%, RSD=3.6%(n=3). CONCLUSIONS The method is simple and accurate. |
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| Keywords: | ferulic acid Shenghua Tang HPLC serum druy concentraion |
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