牙周膜成纤维细胞对成骨细胞细胞数量和碱磷酶活性的影响

目的:观察人牙周膜成纤维细胞(human periodontal ligament fibroblast cells,HPLFs)对成骨细胞(Osteoblast cells,OBs)细胞数量和碱磷酶活性的影响,为进一步探讨正畸牙齿移动的生物学机制奠定基础。方法:建立人OBs与HPLFs共培养系统,通过细胞计数及生化检测法观察人牙周膜成纤维细胞对成骨细胞细胞数量和碱性磷酸酶(alkalinephosphatase,ALP)活性的影响。结果:3d和5d时,共培养组OBs细胞计数分别为4.5×104及8.5×104,明显高于对照组,且两组分别与对照组OBs细胞计数有显著性差异(P<0.05)。transwell共培养组与对照组相比,在3d时,两组OBsALP活性有显著性差异(P<0.05),5d及7d时差异尤其显著(P<0.01),transw ell共培养组OBsALP活性低于对照组。结论:人HPLFs能增加OBs的细胞数量,但抑制OBsALP活性。

The effects of human periodontal ligament fibroblast cells on biological characteristics of human osteoblast cells

Objective： In order to determine the effect of factors pertaining to the development and metabolism of periodontal ligament, the author investigated the effect of human periodontal ligament fibroblast cells（HPLFs） on human Osteoblast cells （OBs）. Methods： The co-culture system of OBs and human HPLFs separated by transwell was established. The proliferation and alkaline phosphatase （ALP） activity of OBs were evaluated by cell number counter and enzyme kinetics methods after 1, 3, 5, 7 days. Results： After co-culture, the number of OBs were 4.5×104 after 3 days and 8.5×104 after 5 days. Compared with the control group, the difference between the two groups and control group was significant （P〈0.05）.The ALPase activities of OBs were reduced by co-culture with human HPLFs after 3, 5, 7 days. Conclusion： Human HPLFs could induce the proliferation of OBs. while restrain the differentiation ability of OBs.