Surface modification of titanium thin film with chitosan via electrostatic self-assembly technique and its influence on osteoblast growth behavior |
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Authors: | Kaiyong Cai Yan Hu Klaus D Jandt Yuanliang Wang |
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Affiliation: | (1) College of Bioengineering, Chongqing University, Chongqing, 400044, P.R. China;(2) Institute of Materials Science & Technology, Friedrich-Schiller-University Jena, Loebdergraben 32, 07743 Jena, Germany |
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Abstract: | Chitosan (Chi) and poly (styrene sulfonate) (PSS) were employed to surface modify titanium thin film via electrostatic self-assembly
(ESA) technique in order to improve its biocompatibility. The surface chemistry, wettability and surface topography of the
coated films with different number of deposited layers were investigated by using X-ray photoelectron spectroscopy (XPS),
water contact angle measurement and atomic force microscopy (AFM), respectively. The results indicated that a full surface
coverage for the outmost layer was achieved at least after deposition of five layers, i.e., PEI/(PSS/Chi)2 on the titanium films. The formed multi-layered structure of PEI(PSS/Chi)
x
(x ≥ 2) on the titanium film was stable in air at room temperature and in phosphate buffered solution (PBS) for at least 3 weeks.
Cell proliferation, cell viability, DNA synthesis as well as differentiation function (alkaline phosphatase) of osteoblasts
on chitosan-modified titanium film (PEI/(PSS/Chi)6) and control sample were investigated, respectively. Osteoblasts cultured on chitosan-modified titanium film displayed a
higher proliferation tendency than that of control (p < 0.01). Cell viability, alkaline phosphatase as well as DNA synthesis measurements indicated that osteoblasts on chitosan-modified
titanium films were greater (p < 0.01) than those for the control, respectively. These results suggest that surface modification of titanium film was successfully
achieved via deposition of PEI/(PSS/Chi)
x
layers, which is useful to enhance the biocompatibility of the titanium film. |
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Keywords: | |
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