不同年龄段大鼠脊髓源性神经干细胞的体外培养及鉴定

背景：不同年龄段神经千细胞的增殖及分化特性目前未见报道。目的：观察不同年龄段人鼠脊髓源性神经干细胞的体外分离、培养及鉴定。方法：取不同年龄大鼠的脊髓，制成单细胞悬液，用含有表皮生长因子、碱性成纤维细胞生长因子和B27的DMEM／F12培养基，以细胞浓度5.0×10^5L^-1进行原代培养，每隔3d传代1次。结果与结论：不同年龄段大鼠的脊髓中均可培养出细胞，其形态为结构紧密、大小不等的悬浮细胞球，称之为“神经干细胞球”；经过传代后单细胞又迅速增殖成球且数目明显增多。提示不同年龄段大鼠脊髓中均能够在体外培养出神经细胞球。

In vitro culture and identification of spinal cord neural stem cells from rats of different ages

BACKGROUND： There have been few reports describing the proliferation and differentiation characteristics of neural stem cells from rats of different ages. OBJECTIVE： To investigate the in vitro isolation, culture and identification of spinal cord neural stem cells from rats of different ages. METHODS： Spinal cord from rats of different ages was taken and prepared into single cell suspension. Primary cells at 5.0×10^5L^-1 were cultured with DMEM/F12 medium containing epidermal growth factor, basic fibroblast growth and B27 and then passaged once every other day. RESULTS AND CONCLUSION： Cells could be cultured from different ages of rat spinal cord. The suspending cell spheres, named neural stem cell spheres showed compact structure and were of different sizes. After passage, single ceils proliferated rapidly and form neurospheres and the number increased obviously. Neural stem cell spheres can be in vitro cultured from the spinal cord of rats at different ages.