Effects of mancozeb and other dithiocarbamate fungicides on <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis>: the role of mitochondrial petite mutants in dithiocarbamate tolerance |
| |
Authors: | E Casalone E Bonelli M Polsinelli |
| |
Affiliation: | Department of Animal Biology and Genetics, University of Florence, 50125 Florence, Italy. enrico.casalone@unifi.it |
| |
Abstract: | Saccharomyces cerevisiae as model system was used to evaluate the occurrence of resistant mutants and adaptation mechanism to mancozeb (MZ), a widespread
fungicide of the dithiocarbamate class with a broad spectrum of action and multiple cell targets. We were unable to isolate
mutants resistant to inhibitory concentration of MZ but found an unusually large number of mitochondrial defective petite
mutants among cells incubated in the presence of subinhibitory MZ concentration. Similar results were obtained with two other
dithiocarbamate fungicides. Comparison of wild type and petite mutants showed that the latter were more resistant to toxic
effects of MZ, highlighting the role of mitochondria in MZ-tolerance. The data suggest that petite cells, arising by exposure
to sub-inhibitory MZ concentration, are not induced by fungicides but are spontaneous mutants already present in the population
before the contact with the fungicide. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|