Mechatronic DNA devices driven by a G-quadruplex-binding platinum ligand |
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Affiliation: | 1. Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada;2. Department of Chemistry, McGill University, Montréal, Québec H3A 2K6, Canada;1. Dept. of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK;1. Tenera Environmental Inc., San Luis Obispo, CA 93401, USA;2. Center for Coastal Marine Sciences, California Polytechnic State University, San Luis Obispo, CA 93407, USA;1. School of Science, Linyi University, Linyi 276005, Shandong, China;2. School of Science, Shandong University of Technology, Zibo 255049, Shandong, China;1. Department of Chemistry, University of Cape Town, Rondebosch 7701, South Africa;2. South African Medical Research Council Drug Discovery and Development Research Unit, University of Cape Town, Rondebosch 7701, South Africa;3. Institute of Infectious Disease & Molecular Medicine, University of Cape Town, Rondebosch 7701, South Africa;4. MRC/NHLS/UCT Molecular Mycobacteriology Research Unit and DST/NRF Centre of Excellence for Biomedical Tuberculosis Research, Department of Clinical Laboratory Sciences, Faculty of Health Sciences, University of Cape Town, Rondebosch 7701, South Africa |
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Abstract: | Contractile duplexes are DNA double helices that incorporate two strategically placed patches of guanine–guanine (G·G) base mismatches. Such duplexes are cation-driven mechatronic devices, able to toggle between states with distinct mechanical and charge conduction properties. In aqueous lithium chloride solution contractile duplexes have an extended (E) and poorly conductive conformation; however, potassium ions drive them to a relatively conductive and structurally contracted (C) conformation, via intramolecular G-quadruplex formation. Here, we report that even in the absence of K+ ions, a known G-quadruplex binding ligand, Pt-PIP phenylphenanthroimidazole ethylenediamine platinum(II)] efficiently promotes the E → C transition, while a poor binder, Pt-bpy bipyridine ethylenediamine platinum(II)], does not promote this transition. An examination of E → C transitions within two different designs for DNA contractile helices found an unexpected complexity: the formation of distinct C states, both electrically conductive, but possessing dissimilar DNA topologies. Ligand-driven DNA mechatronic devices such as these may constitute prototypes for electronic biosensors that identify G-quadruplex binding ligands. |
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Keywords: | DNA G-quadruplex DNA machine DNA conduction Synapsable DNA Contractile switch Phenylphenanthroimidazole ethylenediamine platinum(II) Bipyridine ethylenediamine platinum(II) |
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