| 胆碱酯酶抑制法检测辛硫磷农药残留 |
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| 引用本文： | 侯学文,何衍彪,徐汉虹.胆碱酯酶抑制法检测辛硫磷农药残留[J].河北师范大学学报(自然科学版),2003,27(2):181-184. |
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| 作者姓名： | 侯学文 何衍彪 徐汉虹 |
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| 作者单位： | 华南农业大学,资源环境学院,广东,广州,510642 |
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| 基金项目： | 农业部农药检定所合作推广项目 |
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| 摘 要： | 利用胆碱酯酶水解碘化酰胆碱后形成的硫代胆碱，与二硫双对硝基苯甲酸发生化学反应生成黄色产物，在412nm比色测定。利用此法测定酶、底物的添加量以及保温温度和保温时间对酶活力的影响，还研究了不同浓度与保温时间的辛硫磷对胆碱酯酶活力的抑制情况。获得了胆碱酯酶优化的反应条件，结果还表明胆碱酯酶对辛硫磷比较敏感，其抑制中浓度为I50为3．9049μg/L。
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| 关 键 词： | 胆碱酯酶抑制法 辛硫磷 农药残留检测 酶活力 硫代胆碱 含量测定 |
| 文章编号： | 1000-5854(2003)02-0181-04 |
A Method to Detect Phoxim Residue by Analyzing the Inhibition of Cholinesterase |
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| HOU Xue-wen,HE Yan-biao,XU Han-hong.A Method to Detect Phoxim Residue by Analyzing the Inhibition of Cholinesterase[J].Journal of Hebei Normal University,2003,27(2):181-184. |
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| Authors: | HOU Xue-wen HE Yan-biao XU Han-hong |
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| Abstract: | Ellman reaction is a simple and sensitive method to analyze cholinesterase activity. A new found cholinesterase was studied to get the best reaction condition through Ellman reaction, and it was found this cholinesterase was very sensitive to phoxim, its medium inhibition concentration I 50 to this cholinesterase was only 3.904?9?mg/L, so it had good promise to be used in detecting organophosphate and carbamate pesticide residue in agricultural products. |
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| Keywords: | Ellman reaction phoxim pesticide residue |
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