| 荧光纳米颗粒NaYF_4:Yb,Er作为显像介质的生物相容性 |
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| 引用本文： | 虞永江,马晓荣,于国鹏,高同斌,齐隽,陈方. 荧光纳米颗粒NaYF_4:Yb,Er作为显像介质的生物相容性[J]. 上海交通大学学报(医学版), 2011, 31(1): 15-20. DOI: 10.3969/j.issn.1674-8115.2011.01.004 |
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| 作者姓名： | 虞永江 马晓荣 于国鹏 高同斌 齐隽 陈方 |
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| 作者单位： | 1. 上海交通大学,医学院附属新华医院泌尿外科,上海,200092
2. 上海交通大学附属儿童医院泌尿外科,上海,200040 |
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| 基金项目： | 上海交通大学医学院附属新华医院基金(09QYJ03)~~ |
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| 摘 要： | 目的检测上转频荧光纳米颗粒的生物学体内、外相容性,证实其作为显像介质的生物安全性。方法将培育后的小鼠骨髓间充质干细胞(BMSC)、胚胎成纤维细胞(NIH/3T3)及成肌细胞(C2C12)分别与不同浓度(0、10、50、100、200μg/mL)的NaYF4:Yb,Er共孵育,采用MTT法检测细胞的增殖活性,并测定C2C12成肌细胞形成肌管细胞的功能。将NaYF4:Yb,Er纳米颗粒DMEM混悬液注射入C57BL/6小鼠,行小鼠肝肾功能测定;并对重要脏器行HE组织学染色,检测小鼠的体内毒性。结果 MTT法细胞毒性检测显示,NaYF4:Yb,Er纳米颗粒对NIH/3T3和C2C12的毒性呈剂量与孵育时间正相关性(NIH/3T3:r=0.974,P<0.05;C2C12:r=0.996,P<0.05);而对BMSC的毒性并没有表现出剂量与孵育时间具有相关性(r=-0.218,P>0.05)。NaYF4:Yb,Er纳米颗粒孵育48h后,对C2C12成肌细胞形成肌管细胞功能未见明显影响。小鼠被注射NaYF4:Yb,Er纳米颗粒DMEM混悬液2、4周后,肝肾功能及重要脏器均未见明显损伤。结论 NaYF4:Yb,Er纳米颗...
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| 关 键 词： | 上转频荧光纳米颗粒 NaYF4:Yb Er 生物显像介质 生物相容性 |
Biocompatibility of fluorescent nanoparticles NaYF_4:Yb,Er as imaging media |
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| YU Yong-jiang,MA Xiao-rong,YU Guo-peng,GAO Tong-bin,QI Juan,CHEN Fang. Biocompatibility of fluorescent nanoparticles NaYF_4:Yb,Er as imaging media[J]. Journal of Shanghai Jiaotong University:Medical Science, 2011, 31(1): 15-20. DOI: 10.3969/j.issn.1674-8115.2011.01.004 |
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| Authors: | YU Yong-jiang MA Xiao-rong YU Guo-peng GAO Tong-bin QI Juan CHEN Fang |
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| Affiliation: | YU Yong-jiang1,MA Xiao-rong1,YU Guo-peng1,GAO Tong-bin1,QI Juan1,CHEN Fang2(1.Department of Urology,Xinhua Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200092,China,2.Department of Urology,Shanghai Children's Hospital,Shanghai Jiaotong University,Shanghai 200040,China) |
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| Abstract: | Objective To investigate the biocompatibility of upconversion fluorescent nanoparticles in vivo and in vitro,and verify its safety as imaging media.Methods Mouse bone mesenchymal stem cells(BMSC),mouse embryonic fibroblasts(NIH/3T3) and primary myoblasts(C2C12) were incubated with different concentrations of NaYF4:Yb,Er(0,10,50,100 and 200 μg/mL).Cell proliferation was determined by MTT assay,and the formation of myotube cells from C2C12 myoblasts was detected.DMEM with NaYF4:Yb,Er nanoparticles were inject... |
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| Keywords: | upconversion fluorescent nanoparticles NaYF4:Yb Er biological imaging media biocompatibility |
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