| 过表达miR-26b-5p抑制HMGA1参与卵巢癌细胞上皮间质转化的机制 |
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| 引用本文： | 曹文红. 过表达miR-26b-5p抑制HMGA1参与卵巢癌细胞上皮间质转化的机制[J]. 武警医学, 2019, 30(9): 760-763 |
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| 作者姓名： | 曹文红 |
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| 作者单位： | 266000,青岛市妇女儿童医院妇产科 |
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| 摘 要： | 目的 探究microRNA-26b-5p(miR-26b-5p)介导高迁移率族蛋白组A1(high-mobility group A1,HMGA1)基因参与卵巢癌上皮间质转化的机制。方法 对miR-26b-5p靶基因进行预测。qRT-PCR检测组织中miR-26b-5p和HMGA1的表达量。卵巢癌细胞分组转染后,qRT-PCR和Western Blot检测细胞中相关因子的mRNA和蛋白的表达量;Transwell侵袭实验检测细胞侵袭,细胞划痕实验检测迁移能力。结果 卵巢癌组织中miR-26b-5p表达降低,HMGA1的表达升高(均P＜0.05)。miR-26b-5p mimic及sh-HMGA1组HMGA1和Vimentin表达降低而E-cadherin表达上调,且细胞侵袭和迁移降低(均P＜0.05);共转染miR-26b-5p inhibitor和sh-HMGA1,sh-HMGA1处理对上皮间质转化及细胞侵袭和迁移的抑制作用被逆转(均P＜0.05)。结论 过表达miR-26b-5p可抑制卵巢癌细胞中HMGA1和Vimentin表达并促进E-cadherin表达,降低细胞侵袭和迁移能力,从而抑制卵巢癌细胞上皮间质转化。
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| 关 键 词： | 卵巢癌细胞 miR-26b-5p HMGA1 Vimentin E-cadherin 上皮间质转化 |
| 收稿时间： | 2019-03-25 |
Over-expression of MiR-26b-5p inhibits HMGA1 expression and participates in epithelial-mesenchymal transformation of ovarian cancer cells |
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| CAO Wenhong. Over-expression of MiR-26b-5p inhibits HMGA1 expression and participates in epithelial-mesenchymal transformation of ovarian cancer cells[J]. Medical Journal of the Chinese People's Armed Police Forces, 2019, 30(9): 760-763 |
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| Authors: | CAO Wenhong |
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| Affiliation: | Department of Obstetrics and Gynecology, Women and Children’s Hospital of Qingdao, Qingdao 266000 China |
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| Abstract: | |
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| Keywords: | ovarian cancer cell miR-26b-5p HMGA1 Vimentin E-cadherin epithelial-mesenchymal transformation |
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