耐除草剂玉米G1105E-823C定性检测方法

转基因耐除草剂玉米G1105E-823C是经过改造的转mG2-aroA基因耐草甘膦玉米新品系，具有更高的草甘膦耐受性，目前已完成生产性试验，具有重要的产业化应用前景。但目前尚无针对G1105E-823C的转化体特异性检测方法的相关报道，这十分不利于对该品系的检测及监管。基于此，以G1105E-823C转化体特异性序列为靶标，建立了转基因耐除草剂玉米G1105E-823C的普通PCR和实时荧光PCR定性检测方法。结果表明，2种方法均能检测出转基因耐除草剂玉米G1105E-823C转化体成分，且具有较高的特异性。普通PCR检测方法检出限达0.1%，实时荧光PCR检测方法检出限达0.05%。研究建立的2种定性检测方法为转基因耐除草剂玉米G1105E-823C的精准检测提供了新的技术手段，可为农业转基因监管提供技术支撑。

Qualitative PCR Methods for the Detection of Transgenic Herbicide-tolerant Maize G1105E-823C

Transgenic herbicide-resistant maize G1105E-823C is a new glyphosate-resistant transgenic maize line with modified mG2-aroA gene. It has a higher tolerance to glyphosate-herbicide. At present, this strain has completed production test and has important industrial application prospect in China. However, there is no relevant report on the specific detection method of transformants for G1105E-823C, which is very unfavorable for the detection and supervision of this strain. Based on this, the ordinary PCR and real-time fluorescence PCR qualitative detection methods of transgenic herbicide-resistant maize G1105E-823C were established with the specific sequence of G1105E-823C transformant as the target. The results showed that both methods could detect the components of transgenic herbicide-resistant maize G1105E-823C transformants with high specificity. The detection limit of ordinary PCR detection method was 0.1%, and that of real-time fluorescence PCR detection method was 0.05%. The two qualitative detection methods established in this study provided new technical means for the accurate detection of transgenic herbicide-resistant maize G1105E-823C, and provided technical support for agricultural transgenic supervision.