Prx1调控PINK1/Parkin介导的线粒体自噬在实验性口腔黏膜癌变中的作用

目的 检测口腔黏膜癌变过程中线粒体自噬相关蛋白PINK1、Parkin表达变化及抗氧化蛋白Prx1对其调控作用,探讨Prx1在口腔黏膜癌变中的作用机制.方法 利用4NQO化学诱导Prx1+/+和Prx1+/-小鼠舌黏膜癌变模型,采用免疫组织化学染色及Q-PCR方法 ,检测PINK1、Parkin在小鼠舌正常黏膜、白斑、...

Peroxiredoxin 1 regulates PINK1/Parkin-mediated mitophagy in 4NQO-induced oral carcinogenesis

Objective To examine the expression of PINK1 and Parkin and investigate the regulatory effect of Prx1 on their expression in 4NQO-induced mouse tongue carcinogenesis.Methods 4NQO-induced animal model of tongue mucosa carcinogenesis was established in Prx1^+/+and Prx1+/-mice.The expression of PINK1 and Parkin was examined with immunohistochemistry and Q-PCR at the end of 24 weeks.Results Immunohistochemistry and Q-PCR results showed that in the 4NQO-induced Prx1^+/+mouse tongue carcinogenesis model,the protein and mRNA expression of PINK1 and Parkin was significantly higher in the tongue leukoplakia tissues than those in normal and tongue cancer tissues.Compared with the Prx1^+/+mice,Prx1 knockout significantly reduced PINK1 protein and mRNA expression in tongue leukoplakia,leukoplakia with hyperplasia,and tongue cancer tissues.In addition,the expression of Parkin mRNA in tongue leukoplakia and cancer tissues was significantly increased.Conclusions Mitophagy is involved in oral carcinogenesis and Prx1 may play a key role via regulating PINK1/Parkin-mediated mitophagy in oral cancer progression.