Measurement of total and differential white blood cell counts in synovial fluid by means of an automated hematology analyzer |
| |
Authors: | Sugiuchi Hiroyuki Ando Yukio Manabe Masahiro Nakamura Eiichi Mizuta Hiroshi Nagata Shiro Okabe Hiroaki |
| |
Affiliation: | Department of Laboratory Medicine, Kumamoto University Medical School, Kumamoto, Japan. |
| |
Abstract: | We developed a rapid and accurate method for quantifying total and differential white blood cell (WBC) counts by pretreating synovial fluid with hyaluronidase and using an automated hematology analyzer. Forty-seven samples of synovial fluid that had been placed in blood-collection tubes containing ethylenediamine- N,N,N',N' -tetraacetic acid as an anticoagulant were treated with hyaluronidase at 37 degrees C for 10 minutes, and then the total and differential WBC counts were determined by means of the automated hematology analyzer. Results were compared with those achieved by traditional manual counting methods. For the automated method, the coefficient of variation values for within-run precision of the WBC count were 5.27%, 3.56%, and 3.01% at 0.54, 1.12, and 2.05 x 10(9) /L, respectively; the run-to-run coefficient of variation values was less than 10.0%. The total and differential WBC counts obtained by this automated method showed good correlation with those obtained by the hemocytometer method ( r = .998; P < .0001; regression formula, y = 0.986 x - 0.072). Bland-Altman plots indicated no significant discrepancy between the methods. Our evaluation supports the use of this automated hematology analyzer method to measure total and differential WBC counts, which should aid clinical diagnosis. |
| |
Keywords: | WBC white blood cell PMN polymorphonuclear neutrophil leukocyte CV coefficient of variation DIFF differential BASO basophil |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|