| SPE-HPLC法测定胃肠宁片中3种生物碱的含量 |
| |
| 引用本文： | 丘明明,黄玉华.SPE-HPLC法测定胃肠宁片中3种生物碱的含量[J].中成药,2011,33(2):276-279. |
| |
| 作者姓名： | 丘明明 黄玉华 |
| |
| 作者单位： | 广西食品药品检验所,广西南宁,530021 |
| |
| 摘 要： | 目的:建立SPE-HPLC法测定胃肠宁片(布渣叶,辣蓼,番石榴叶,火炭母,功劳木)中盐酸药根碱、盐酸巴马汀和盐酸小檗碱3种生物碱的含量.方法:选用WCX弱阳离子交换柱用于固相萃取,HPLC采用Hvydro-RP 80A C18柱(250 mm ×4.6 mm,5μm),乙腈-0.05 mol/L磷酸二氢钠溶液(磷酸调pH2.8)(27:73)为流动相,柱温为35℃,流速:1.0 mL/min,检测波长为265 nm.结果:盐酸药根碱、盐酸巴马汀、盐酸小檗碱分别在3.443 7～6.887 4 ng(r=1.000 0)、2.306～23.060 ng(r=1.000 0)和2.361 3～23.613 5 ng(r=0.999 7)范围内线性关系良好,回收率分别为99.71%(RSD为0.85%)、100.32%(RSD为0.98%)和100.32%(RSD为1.09%)(n=9).结论:该方法操作简便,准确性、重复性好,可用于胃肠宁片中盐酸小檗碱、盐酸巴马汀和盐酸药根碱的含量测定.
|
| 关 键 词： | 固相萃取 高效液相色谱法 胃肠宁片 盐酸小檗碱 盐酸巴马汀 盐酸药根碱 |
Determination of three alkaloids in Weichangning Tablet by SPE-HPLC |
| |
| QIU Ming-ming
,
HUANG Yu-hua.Determination of three alkaloids in Weichangning Tablet by SPE-HPLC[J].Chinese Traditional Patent Medicine,2011,33(2):276-279. |
| |
| Authors: | QIU Ming-ming HUANG Yu-hua |
| |
| Affiliation: | QIU Ming-ming,HUANG Yu-hua(Guangxi Institute for Food and Drug Control,Nanning 530021,China) |
| |
| Abstract: | |
| |
| Keywords: | solid phase extraction HPLC Weichangning Tablet jatrorrhizine hydrochloride palmatine hydrochloride berberine hydrochloride |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
